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Literature summary extracted from

  • Hu, Z.; Plaxton, W.C.
    Purification and characterization of cytosolic pyruvate kinase from leaves of the castor oil plant (1996), Arch. Biochem. Biophys., 333, 298-307.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 citrate
-
 Ricinus communis
2.7.1.40 L-glutamate
-
 Ricinus communis
2.7.1.40 oxalate
-
 Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.033
-
 phosphoenolpyruvate pH 7.5, 30°C Ricinus communis
2.7.1.40 0.037
-
 ADP pH 7.5, 30°C Ricinus communis
2.7.1.40 0.037
-
 ADP ADP in form of MgADP- Ricinus communis
2.7.1.40 0.087
-
 ADP pH 6.5, 30°C Ricinus communis
2.7.1.40 0.087
-
 ADP ADP in form of MgADP- Ricinus communis
2.7.1.40 0.089
-
 phosphoenolpyruvate pH 6.5, 30°C Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 cytosol
-
 Ricinus communis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Ricinus communis
2.7.1.40 Mg2+ requirement Ricinus communis
2.7.1.40 additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 56000
-
 2 * 56000 + 2 * 57000, SDS-PAGE Ricinus communis
2.7.1.40 57000
-
 2 * 56000 + 2 * 57000, SDS-PAGE Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Ricinus communis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 leaf
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 51
-
 pH 6.5, 30°C Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Ricinus communis ATP + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 2 * 56000 + 2 * 57000, SDS-PAGE Ricinus communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 55
-
 5 min, 10% loss of activity Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.5
-
 broad Ricinus communis