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Literature summary extracted from

  • Singh, D.K.; Malhotra, S.P.; Singh, R.
    Purification and characterization of cytosolic pyruvate kinase from developing seeds of Brassica campestris L (2000), Indian J. Biochem. Biophys., 37, 51-58.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate
-
 Brassica rapa subsp. oleifera
2.7.1.40 D-glucose 6-phosphate
-
 Brassica rapa subsp. oleifera
2.7.1.40 phosphate
-
 Brassica rapa subsp. oleifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 citrate
-
 Brassica rapa subsp. oleifera
2.7.1.40 fumarate
-
 Brassica rapa subsp. oleifera
2.7.1.40 glutamine
-
 Brassica rapa subsp. oleifera
2.7.1.40 isocitrate
-
 Brassica rapa subsp. oleifera
2.7.1.40 L-glutamate
-
 Brassica rapa subsp. oleifera
2.7.1.40 oxalate
-
 Brassica rapa subsp. oleifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.1
-
 phosphoenolpyruvate pH 6.8, 30°C Brassica rapa subsp. oleifera
2.7.1.40 0.11
-
 ADP pH 6.8, 30°C Brassica rapa subsp. oleifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 cytosol
-
 Brassica rapa subsp. oleifera 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ fulfills requirement for monovalent cation Brassica rapa subsp. oleifera
2.7.1.40 Mg2+ fulfills absolute requirement for divalent cation Brassica rapa subsp. oleifera
2.7.1.40 additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Brassica rapa subsp. oleifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 55000
-
 x * 55000 + 4-x * 57000, SDS-PAGE Brassica rapa subsp. oleifera
2.7.1.40 57000
-
 x * 55000 + 4-x * 57000, SDS-PAGE Brassica rapa subsp. oleifera
2.7.1.40 214000
-
 gel filtration Brassica rapa subsp. oleifera

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Brassica rapa subsp. oleifera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Brassica rapa subsp. oleifera

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate compulsory-ordered tri-bi mechanism Brassica rapa subsp. oleifera
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 seed
-
 Brassica rapa subsp. oleifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate poor substrates: UDP, GDP Brassica rapa subsp. oleifera ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Brassica rapa subsp. oleifera ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer x * 55000 + 4-x * 57000, SDS-PAGE Brassica rapa subsp. oleifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8
-
-
 Brassica rapa subsp. oleifera