Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tanaka, K.; Sakai, H.; Ohta, T.; Matsuzawa, H.
    Molecular cloning of the genes for pyruvate kinase of two bacilli, Bacillus psychrophilus and Bacillus licheniformis, and comparison of the properties of the enzymes produced in Escherichia coli (1995), Biosci. Biotechnol. Biochem., 59, 1536-1542.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 AMP
-
 Sporosarcina psychrophila
2.7.1.40 AMP
-
 Bacillus licheniformis
2.7.1.40 D-ribose 5-phosphate
-
 Escherichia coli
2.7.1.40 D-ribose 5-phosphate
-
 Sporosarcina psychrophila
2.7.1.40 D-ribose 5-phosphate
-
 Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40
-
 Sporosarcina psychrophila
2.7.1.40
-
 Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP
-
 Bacillus licheniformis
2.7.1.40 ATP
-
 Sporosarcina psychrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 61940
-
 x * 61940, deduced from gene sequence Bacillus licheniformis
2.7.1.40 62570
-
 x * 62570, deduced from gene sequence Sporosarcina psychrophila
2.7.1.40 220000
-
 gel filtration Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Bacillus licheniformis P51181
-
-
2.7.1.40 Escherichia coli
-
-
-
2.7.1.40 Sporosarcina psychrophila P51182
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme Sporosarcina psychrophila
a
2.7.1.40 ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme Bacillus licheniformis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 175
-
 30°C Bacillus licheniformis
2.7.1.40 202
-
 30°C Sporosarcina psychrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Escherichia coli ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Sporosarcina psychrophila ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Bacillus licheniformis ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 ? x * 62570, deduced from gene sequence Sporosarcina psychrophila
2.7.1.40 ? x * 61940, deduced from gene sequence Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 50
-
-
 Sporosarcina psychrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.6
-
 30°C Sporosarcina psychrophila