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Literature summary extracted from

  • Smith, C.R.; Knowles, V.L.; Plaxton, W.C.
    Purification and characterization of cytosolic pyruvate kinase from Brassica napus (rapeseed) suspension cell cultures: implications for the integration of glycolysis with nitrogen assimilation (2000), Eur. J. Biochem., 267, 4477-4485.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 L-aspartate Ka-value 0.31 mM, reverses inhibition by L-glutamate Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 L-glutamate 50% inhibition at 4 mM Brassica napus
2.7.1.40 oxalate 50% inhibition at 4 mM Brassica napus
2.7.1.40 quercetin 50% inhibition at 0.1 mM Brassica napus
2.7.1.40 rutin 50% inhibition at 0.07 mM Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
additional information hyperbolic saturation kinetics for phosphoenolpyruvate, ADP, Mg2+, K+ Brassica napus
2.7.1.40 0.075
-
ADP pH 6.8, 24°C Brassica napus
2.7.1.40 0.12
-
phosphoenolpyruvate pH 6.8, 24°C Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 cytosol
-
Brassica napus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ apparent Km-value: 0.48 mM Brassica napus
2.7.1.40 K+ only together with activating divalent cation Brassica napus
2.7.1.40 Mg2+ apparent Km-value: 0,21 mM Brassica napus
2.7.1.40 Mg2+ fulfills absolute requirement for divalent cation Brassica napus
2.7.1.40 additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 56000
-
4 * 56000, SDS-PAGE Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Brassica napus
-
PKc, cytosolic form
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
Brassica napus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 51
-
pH 6.8, 24°C Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Brassica napus ATP + pyruvate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 56000, SDS-PAGE Brassica napus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 65
-
3 min, stable Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8
-
broad Brassica napus