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Literature summary extracted from

  • Potter, S.; Fothergill-Gilmore, L.A.
    Purification and properties of pyruvate kinase from Thermoplasma acidophilum (1992), FEMS Microbiol. Lett., 94, 235-240.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.40 freezing inactivates Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Thermoplasma acidophilum
2.7.1.40 0.04 0.063 phosphoenolpyruvate
-
 Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 250000
-
 PAGE Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Thermoplasma acidophilum
-
 archaebacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 at 45-50°C Thermoplasma acidophilum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Thermoplasma acidophilum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 201
-
 pH 7.5, 60°C Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Thermoplasma acidophilum ATP + pyruvate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 60
-
 assay at Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 40
-
 negligible activity below, reversible at room temperature, irreversible at freezing temperatures Thermoplasma acidophilum
2.7.1.40 70
-
 30 min stable Thermoplasma acidophilum
2.7.1.40 90
-
 10 min stable Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7.5
-
-
 Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP activation Thermoplasma acidophilum