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Literature summary extracted from

  • Garcia-Olalla, C.; Garrido-Pertierra, A.
    Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium (1987), Biochem. J., 241, 573-581.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 D-fructose 1,6-diphosphate kinetics Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.16 0.17 ADP isozyme II, 30°C, pH 6.8 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 0.2 0.35 ADP enzyme form I Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 0.2 0.35 ADP 30°C, pH 6.8 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Mg2+ requirement Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 Mg2+ positive cooperativity Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 Mn2+ requirement Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 47000
-
 4 * 47000, isozyme II, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 54000
-
 4 * 54000, isozyme I, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 190000
-
 isozyme PKII, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 220000
-
 isozyme PKII, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strain LT-2, highest activity in cells grown on glycolytic substrates, e.g. glucose, galactose, fructose or glycerol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 2 isoforms, I and II Salmonella enterica subsp. enterica serovar Typhimurium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, in 50 mM Tris-HCl buffer, isozyme I up to 10 days, isozyme II 4 days Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 EDTA, 2-mercaptoethanol, dithiothreitol, glycerol and KCl stabilize, being more effective at refrigerated conditions than at room temperature Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 frozen, in 50 mM Tris-HCl buffer, isozyme I at least 9 months, isozyme II 4 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Salmonella enterica subsp. enterica serovar Typhimurium ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 54000, isozyme I, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 tetramer 4 * 47000, isozyme II, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 30
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 25
-
 at pH 7-8, stable Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 37
-
 at pH 7-8, stable Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.40 55
-
 15 min, 12% loss of activity, isozyme I, inactivation of isozyme II Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.8
-
 more active in HEPES than in other buffers Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 4 6.8 increase of activity in this range, decline at higher pH-values Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.40 7 8 at 25°C and 37°C, stable Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP activation Salmonella enterica subsp. enterica serovar Typhimurium