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Literature summary extracted from

  • Baysdorfer, C.; Bassham, J.A.
    Spinach pyruvate kinase isoforms. Partial purification and regulatory properties (1984), Plant Physiol., 74, 374-379.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 asparagine activation Spinacia oleracea
2.7.1.40 asparagine synergistic activation with fructose 1,6-diphosphate of isozyme PK-aerobic, not PK-anoxic Spinacia oleracea
2.7.1.40 D-fructose 1,6-diphosphate not Spinacia oleracea
2.7.1.40 D-fructose 2,6-diphosphate not Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-oxoglutarate not Spinacia oleracea
2.7.1.40 adenine weak Spinacia oleracea
2.7.1.40 citrate isozyme PKI, kinetics; not isozyme PKII Spinacia oleracea
2.7.1.40 L-glutamate not isozyme PKI; strong, isozyme PKII, kinetics Spinacia oleracea
2.7.1.40 oxalate kinetics Spinacia oleracea
2.7.1.40 tryptophan weak Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Saccharomyces cerevisiae
2.7.1.40 additional information
-
 additional information kinetic parameters Spinacia oleracea
2.7.1.40 0.72 0.73 UDP pH 7.1, 25°C Spinacia oleracea
2.7.1.40 1.1
-
 GDP pH 7.1, 25°C Spinacia oleracea
2.7.1.40 6.8
-
 CDP isozyme PKII, pH 7.1, 25°C Spinacia oleracea
2.7.1.40 9
-
 CDP isozyme PKI, pH 7.1, 25°C Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+
-
 Saccharomyces cerevisiae
2.7.1.40 K+
-
 Spinacia oleracea
2.7.1.40 Mg2+
-
 Saccharomyces cerevisiae
2.7.1.40 Mg2+
-
 Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Saccharomyces cerevisiae
-
-
-
2.7.1.40 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 partial, 2 isoforms, separable by blue-agarose chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Saccharomyces cerevisiae
2.7.1.40 additional information
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 -20°C, in the absence of substrates, 70% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it Spinacia oleracea
2.7.1.40 -80°C, in the absence of substrates, 95% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it Spinacia oleracea
2.7.1.40 4°C, in the absence of substrates, t1/2: 1 month Spinacia oleracea
2.7.1.40 8°C, isozyme PKI in the absence of substrates, less than 20% loss of activity within 1 month and isozyme PKII with t1/2 of 5 days, 25% loss within 1 month in the presence of phosphoenolpyruvate or 60% loss of activity in the presence of ADP Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Saccharomyces cerevisiae ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Spinacia oleracea ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea GTP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea UTP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 additional information
-
 cold-labile enzyme Saccharomyces cerevisiae
2.7.1.40 additional information
-
 cold-labile enzyme Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 5.5 7.5 about 80% of maximal activity at pH 5.5 and pH 7.5 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP activation Spinacia oleracea
2.7.1.40 AMP not at physiological concentrations Spinacia oleracea