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Literature summary extracted from

  • Ireland, R.J.; De Luca, V.; Dennis, D.T.
    Characterization and kinetics of isoenzymes of pyruvate kinase from developing castor bean endosperm (1980), Plant Physiol., 65, 1188-1193.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.1.40 cytosolic isozyme is much more stable than plastid isozyme, dithiothreitol plus glycerol stabilize plastid isozyme PKp during purification, isozyme PKp requires dithiothreitol or 2-mercaptoethanol during purification, cytosolic isozyme PKc is stable to dilution into 50 mM N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9 Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 citrate
-
 Ricinus communis
2.7.1.40 pyruvate product inhibition Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic parameters Ricinus communis
2.7.1.40 0.03 0.05 ADP 25°C, pH 7.9 Ricinus communis
2.7.1.40 0.04 0.063 phosphoenolpyruvate 25°C, pH 7.9 Ricinus communis
2.7.1.40 0.04 0.063 phosphoenolpyruvate 2 isozymes with different kinetic mechanisms Ricinus communis
2.7.1.40 0.049 0.1 IDP 25°C, pH 7.9 Ricinus communis
2.7.1.40 0.049 0.1 IDP cosubstrate phosphoenolpyruvate Ricinus communis
2.7.1.40 0.12
-
 ADP cosubstrate UDP, isozyme PKc, 25°C, pH 7.9 Ricinus communis
2.7.1.40 0.2 0.35 ADP 25°C, pH 7.9 Ricinus communis
2.7.1.40 0.2 0.35 ADP PKp-isozyme Ricinus communis
2.7.1.40 0.25 0.26 GDP IDP, isozyme PKc, 25°C, pH 7.9 Ricinus communis
2.7.1.40 2.2
-
 GDP isozyme PKp, 25°C, pH 7.9 Ricinus communis
2.7.1.40 2.4
-
 UDP isozyme PKp, 25°C, pH 7.9 Ricinus communis
2.7.1.40 4
-
 IDP isozyme PKp, 25°C, pH 7.9 Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 cytosol 2 isozymes: a cytosolic and a plastid one Ricinus communis 5829
-
2.7.1.40 plastid 2 isozymes: a cytosolic and a plastid one Ricinus communis 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ fulfills requirement for monovalent cation Ricinus communis
2.7.1.40 Mg2+ requirement Ricinus communis
2.7.1.40 Mg2+ Km-value 0.45 mM for isozyme PKc, 1.6 mM for isozyme PKp Ricinus communis
2.7.1.40 Mg2+ fulfills absolute requirement for divalent cation Ricinus communis
2.7.1.40 Mn2+ requirement Ricinus communis
2.7.1.40 Mn2+ fulfills absolute requirement for divalent cation Ricinus communis
2.7.1.40 Mn2+ Km-value 0.05 mM for isozyme PKc, 0.5 mM for isozyme PKp Ricinus communis
2.7.1.40 NH4+ requirement Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Ricinus communis
-
 castor bean, var. Baker 296
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 partial, several isozymes Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 endosperm developing Ricinus communis
-
2.7.1.40 seed
-
 Ricinus communis
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 5°C, isozyme PKp in 50 mM phosphate buffer, pH 7, 50%, v/v, glycerol, 2 mM dithiothreitol, several months and isozyme PKc in 50 mM phosphate buffer, pH 7, at least 1 week Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Ricinus communis ATP + pyruvate
-
 ?
2.7.1.40 CDP + phosphoenolpyruvate less effective than ADP Ricinus communis CTP + pyruvate
-
 ?
2.7.1.40 GDP + phosphoenolpyruvate less effective than ADP Ricinus communis GTP + pyruvate
-
 ?
2.7.1.40 IDP + phosphoenolpyruvate less effective than ADP Ricinus communis ITP + pyruvate
-
 ?
2.7.1.40 UDP + phosphoenolpyruvate less effective than ADP Ricinus communis UTP + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Ricinus communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 25
-
 in N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9, 1% glycerol, 0.04 mM DTT, isozyme PKp: t1/2: 1.5 min, ADP and MgCl2 preserve activity over 10 min, isozyme PKc: at least 30 min Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 6.9 7.2 isozyme PKc Ricinus communis
2.7.1.40 7.8
-
 isozyme PKp Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 6 8.3 isozyme PKc, about half-maximal activity at pH 6 and pH 8.3 Ricinus communis
2.7.1.40 7.2 8.3 isozyme Pkp, about half-maximal activity at pH 7.2 and pH 8.3 Ricinus communis