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Literature summary extracted from

  • Malcovati,M.; Valentini, G.
    AMP- and fructose 1,6-bisphosphate-activated pyruvate kinases from Escherichia coli (1982), Methods Enzymol., 90, 170-179.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Escherichia coli
2.7.1.40 D-glucose 6-phosphate isozyme PK II Escherichia coli
2.7.1.40 D-ribose 5-phosphate activation Escherichia coli
2.7.1.40 D-ribose 5-phosphate isozyme PK II Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 51000
-
 4 * 51000, isozyme PK I, SDS-PAGE Escherichia coli
2.7.1.40 56000
-
 4 * 56000, isozyme PK II, SDS-PAGE Escherichia coli
2.7.1.40 190000
-
 isozyme PK II Escherichia coli
2.7.1.40 225000 240000 isozyme PK I Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Escherichia coli
-
 and strain B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 2 non-interconvertible forms, isozyme PK I: at room temperature Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 0-4°C, in 10 mM Tris, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 1-10 mg isozyme PK I/ml, up to 9 months, in 10 mM Tris, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.15 M KCl, pH 8, 0.5-1 mg isozyme PK II/ml, up to 6 months Escherichia coli
2.7.1.40 K+ stabilize during storage, NH4+ or Na+ less efficiently Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Escherichia coli ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 51000, isozyme PK I, SDS-PAGE Escherichia coli
2.7.1.40 tetramer 4 * 56000, isozyme PK II, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 55
-
 in 5 mM phosphate buffer, pH 7.5, isozyme PKI: t1/2: more than 1 h, isozyme PKII: t1/2: 2 min Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ATP activation Escherichia coli
2.7.1.40 ATP isozyme PK II Escherichia coli