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Literature summary extracted from

  • Murao, S.; Nakatani, A.; Kaneda, N.
    Isolation and characterization of fructokinase from Pseudomonas sp. KN-21 (1995), Biosci. Biotechnol. Biochem., 59, 1798-1800.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.7
-
 ATP pH 7.6, 45°C Pseudomonas sp.
2.7.1.4 0.7
-
 fructose pH 7.6, 45°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.4 Mg2+
-
 Pseudomonas sp.
2.7.1.4 Mn2+ can partially replace Mg2+ in activation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 37000
-
 2 * 37000, SDS-PAGE Pseudomonas sp.
2.7.1.4 75000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Pseudomonas sp.
-
 ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose Pseudomonas sp. KN-21
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Pseudomonas sp.
-
 KN-21
-
2.7.1.4 Pseudomonas sp. KN-21
-
 KN-21
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
2.7.1.4 5°C, stable for 6 weeks at pH 8 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Pseudomonas sp. ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ATP + D-fructose
-
 Pseudomonas sp. KN-21 ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 CTP + D-fructose 40% of the activity with ATP Pseudomonas sp. CDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 dATP + D-fructose 90% of the activity with ATP Pseudomonas sp. dADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 dATP + D-fructose 90% of the activity with ATP Pseudomonas sp. KN-21 dADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose 30% of the activity with ATP Pseudomonas sp. GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose 30% of the activity with ATP Pseudomonas sp. KN-21 GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ITP + D-fructose 25% of the activity with ATP Pseudomonas sp. IDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 ITP + D-fructose 25% of the activity with ATP Pseudomonas sp. KN-21 IDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose 80% of the activity with ATP Pseudomonas sp. UDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose 80% of the activity with ATP Pseudomonas sp. KN-21 UDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 dimer 2 * 37000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.4 45
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 7.6
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.4 6.5 8.5 stable Pseudomonas sp.