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Literature summary extracted from

  • Martinez-Barajas, E.; Randall, D.D.
    Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum) fruits (1996), Planta, 199, 451-458.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 ADP inhibition is dependent on fructose concentration Solanum lycopersicum
2.7.1.4 D-fructose above 0.5 mM Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.11
-
 ATP FKI, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.131
-
 D-fructose FKI, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.15
-
 ATP FKII, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.201
-
 D-fructose FKII, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.69
-
 GTP FKI, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.9
-
 GTP FKII, pH 8, 25°C Solanum lycopersicum
2.7.1.4 1.18
-
 UTP FKI, pH 8, 25°C Solanum lycopersicum
2.7.1.4 1.51
-
 UTP FKII, pH 8, 25°C Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 35000
-
 2 * 35000, SDS-PAGE Solanum lycopersicum
2.7.1.4 73000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.4 ATP + D-fructose Solanum lycopersicum
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4 400fold, FKI and FKII Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.4 fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 24.2
-
 FKII Solanum lycopersicum
2.7.1.4 25
-
 FKI Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Solanum lycopersicum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose
-
 Solanum lycopersicum GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose
-
 Solanum lycopersicum UDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 dimer 2 * 35000, SDS-PAGE Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 8 9.5
-
 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.4 1.39
-
 D-fructose FKII, pH 8, 25°C Solanum lycopersicum
2.7.1.4 1.82
-
 D-fructose FKI, pH 8, 25°C Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.4 Solanum lycopersicum 4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE 6.55 6.07
2.7.1.4 Solanum lycopersicum 2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE 6.55 6.42