Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, K.X.; Scott, A.I.; Bennett, G.N.
    Overexpression, purification, and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii (1999), Protein Expr. Purif., 17, 33-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 overexpression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 farnesyl diphosphate 0.01 mM, 65% inhibition Methanocaldococcus jannaschii
2.7.1.36 geranyl diphosphate 0.01 mM, 55% inhibition Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.069
-
(R,S)-mevalonate pH 8.0, 70°C, recombinant mevalonate kinase Methanocaldococcus jannaschii
2.7.1.36 0.092
-
ATP pH 8.0, 70°C, recombinant mevalonate kinase Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 37000
-
2 * 37000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.36 68000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + mevalonate Methanocaldococcus jannaschii involved in mevalonate pathway of terpenoid biosynthesis ADP + phosphomevalonate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant mevalonate kinase, 70°C heat treatment, Talon metal-affinity resin Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 387
-
recombinant mevalonate kinase Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate
-
Methanocaldococcus jannaschii ADP + phosphomevalonate
-
?
2.7.1.36 ATP + mevalonate involved in mevalonate pathway of terpenoid biosynthesis Methanocaldococcus jannaschii ADP + phosphomevalonate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 dimer 2 * 37000, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.36 70 75
-
Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.36 37 90 approx. 25% of maximal activity at 40°C and 90°C, respectively Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.36 70
-
no loss of activity after 24 h Methanocaldococcus jannaschii
2.7.1.36 90
-
50% loss of activity after 15 min Methanocaldococcus jannaschii
2.7.1.36 100
-
50% loss of activity after 5 min Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.36 8 8.5
-
Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.36 5 7 at least 60% of maximal activity between pH 5.0 and 7.0 Methanocaldococcus jannaschii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.36 Methanocaldococcus jannaschii MonoP chromatography
-
7.8
2.7.1.36 Methanocaldococcus jannaschii calculated from amino acid sequence
-
8.4