Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Falk, K.L.; Guerra, D.J.
    Coenzyme A biosynthesis in plants: partial purification and characterization of pantothenate kinase from spinach (1993), Arch. Biochem. Biophys., 301, 424-430.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.33 Acyl carrier protein PAK II: inhibition, PAK I: stimulation between 0.015-0.035 mM Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 acetyl-CoA not Brassica napus
2.7.1.33 acetyl-CoA not Spinacia oleracea
2.7.1.33 Acyl carrier protein
-
 Brassica napus
2.7.1.33 Acyl carrier protein PAK II: inhibition, PAK I: stimulation between 0.015-0.035 mM Spinacia oleracea
2.7.1.33 CoA not Brassica napus
2.7.1.33 CoA not Spinacia oleracea
2.7.1.33 malonyl-CoA
-
 Brassica napus
2.7.1.33 malonyl-CoA 0.075 mM, complete inhibition of PAK I Spinacia oleracea
2.7.1.33 additional information not inhibitory: 3',5'-ADP; not inhibitory: CoASH, acetyl-CoA Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.8
-
 pantothenate isoform 2, 37°C, pH 8.0 Spinacia oleracea
2.7.1.33 1.3
-
 pantothenate isoform 1, 37°C, pH 8.0 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.33 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ requirement, active substrate: MgATP-complex Spinacia oleracea
2.7.1.33 Mg2+ requirement, active substrate: MgATP-complex Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + D-pantothenate Spinacia oleracea
-
 ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate Brassica napus
-
 ADP + D-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Brassica napus
-
-
-
2.7.1.33 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 partial, presumably 2 isoforms Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.33 leaf
-
 Spinacia oleracea
-
2.7.1.33 seedling
-
 Spinacia oleracea
-
2.7.1.33 seedling
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 0.152
-
 isoform 1, 37°C, pH 8.0 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.33 4°C, t1/2: 3 days Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + D-pantothenate
-
 Spinacia oleracea ADP + D-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + D-pantothenate
-
 Brassica napus ADP + D-4'-phosphopantothenate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 8
-
 broad Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.33 6 9
-
 Spinacia oleracea