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Literature summary extracted from

  • Setty, P.N.; Krishnan, P.S.
    Choline kinase in Cuscuta reflexa (1972), Biochem. J., 126, 313-324.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 ADP
-
 Cuscuta reflexa
2.7.1.32 AMP
-
 Cuscuta reflexa
2.7.1.32 ATP at 4.9 mM and 8.0 mM 30% inhibition; optimum concentration: 3.2-4.0 mM Cuscuta reflexa
2.7.1.32 methionine
-
 Cuscuta reflexa
2.7.1.32 NaCl 8.7-35 mM: slight activation, 70 mM: inactivation Cuscuta reflexa
2.7.1.32 phosphorylcholine
-
 Cuscuta reflexa
2.7.1.32 serine
-
 Cuscuta reflexa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 additional information
-
 additional information
-
 Cuscuta reflexa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 mitochondrion
-
 Cuscuta reflexa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Na+ 8.7-35 mM: slight activation, 70 mM: inactivation Cuscuta reflexa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Cuscuta reflexa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32
-
 Cuscuta reflexa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.32 17.9
-
-
 Cuscuta reflexa

Storage Stability

EC Number Storage Stability Organism
2.7.1.32 -18°C, enzyme kept frozen for 3 h and thawed, 15 min in contact with ice, complete inactivation, cannot be reactivated Cuscuta reflexa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Cuscuta reflexa ADP + O-phosphocholine
-
 ?
2.7.1.32 GTP + choline at 50% of the activity with ATP Cuscuta reflexa GDP + choline phosphate
-
 ?
2.7.1.32 UTP + choline at 20% of the activity with ATP Cuscuta reflexa UDP + choline phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.32 30
-
 assay at Cuscuta reflexa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 8.5
-
-
 Cuscuta reflexa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.32 7.5 9 pH 7.5: about 30% of maximum activity, pH 9.0: about 45% of maximum activity Cuscuta reflexa