EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | ethanolamine | weak competitive inhibitor | Rattus norvegicus | |
2.7.1.82 | ATP | concentration exceeding that of Mg2+ | Rattus norvegicus | |
2.7.1.82 | choline | very strong | Rattus norvegicus | |
2.7.1.82 | Mn2+ | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | additional information | - |
additional information | Km of MgATP2-: 10 mM, in presence of equimolar amounts of ATP and Mg2+, 1.5 mM, in 2.5-fold higher concentration of Mg2+ than ATP | Rattus norvegicus | |
2.7.1.82 | additional information | - |
additional information | - |
Rattus norvegicus | |
2.7.1.82 | 1.5 | - |
ATP | with 1.5-fold higher concentration of Mg2+ than ATP, pH 8.5 | Rattus norvegicus | |
2.7.1.82 | 1.5 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
2.7.1.82 | 10 | - |
ATP | in presence of equivalent amounts of ATP and Mg2+, pH 8.5 | Rattus norvegicus | |
2.7.1.82 | 10 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.32 | cytosol | - |
Rattus norvegicus | 5829 | - |
2.7.1.82 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | Mg2+ | ATP concentration 1.25-2.5 mM: reaction requires free Mg2+ rather than MgATP2-, ATP concentration exceeding that of Mg2+: strong inhibition | Rattus norvegicus | |
2.7.1.32 | Mg2+ | Km MgATP2-: 10 mM, in presence of equimolar amounts of ATP and Mg2+, Km MgATP2-: 1.5 mM, in 2.5 fold higher concentration of Mg2+ than ATP | Rattus norvegicus | |
2.7.1.82 | Mg2+ | Km MgATP2-: 10 mM, in presence of equivalent amounts of ATP and Mg2+ | Rattus norvegicus | |
2.7.1.82 | Mg2+ | 1.25-2.5 mM ATP: reaction requires free Mg2+ rather than an ATP-Mg2+ complex for maximal velocity | Rattus norvegicus | |
2.7.1.82 | Mg2+ | 1.5 mM, in 1.5-fold higher concentration of Mg2+ than ATP | Rattus norvegicus | |
2.7.1.82 | Mg2+ | ATP concentration exceeding that of Mg2+: strong inhibition | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Rattus norvegicus | - |
- |
- |
2.7.1.82 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.32 | copurification of choline kinase and ethanolamine kinase | Rattus norvegicus |
2.7.1.82 | copurification of choline kinase and ethanolamine kinase | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.32 | ATP + choline = ADP + phosphocholine | choline kinase and ethanolamine kinase may not have a common active site in a single enzyme protein | Rattus norvegicus | |
2.7.1.82 | ATP + ethanolamine = ADP + O-phosphoethanolamine | choline kinase and ethanolamine kinase may not have a common active site in a single enzyme protein | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.82 | kidney | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 1.5 | - |
- |
Rattus norvegicus |
2.7.1.82 | 1.398 | - |
pH 8.5 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | ATP + choline | - |
Rattus norvegicus | ADP + O-phosphocholine | - |
? | |
2.7.1.82 | ATP + ethanolamine | - |
Rattus norvegicus | ADP + O-phosphoethanolamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.82 | More | cf. EC 2.7.1.32 | Rattus norvegicus |
2.7.1.82 | More | choline kinase and ethanolamine kinase may not have a common active site in a single enzyme protein | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 8.5 | 9 | - |
Rattus norvegicus |
2.7.1.82 | 8 | 9 | - |
Rattus norvegicus |