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Literature summary extracted from

  • Kleczkowski, L.A.; Randall, D.D.
    Purification and partial characterization of spinach leaf glycerate kinase (1983), FEBS Lett., 158, 313-316.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.31 additional information
-
 additional information
-
 Spinacia oleracea
2.7.1.31 0.285
-
 D-glycerate 25°C, pH 7.8 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.31 39500
-
 1 * 39500, SDS-PAGE Spinacia oleracea
2.7.1.31 40000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.31 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.31
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.31 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.31 additional information
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.31 purified enzyme totally unstable after overnight storage in a freezer unless bovine serum albumin, 3 mg/ml, is added Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.31 ATP + (R)-glycerate specific for D-isomer Spinacia oleracea ADP + 3-phospho-(R)-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.31 monomer 1 * 39500, SDS-PAGE Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.31 25
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.31 6.5 8.5
-
 Spinacia oleracea