Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Doughty, C.C.; Hayashi, J.A.; Guenther, H.L.
    Purification and properties of D-glycerate 3-kinase from Escherichia coli (1966), J. Biol. Chem., 241, 568-572.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.31
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.31 EDTA
-
 Escherichia coli
2.7.1.31 iodoacetate
-
 Escherichia coli
2.7.1.31 additional information 60% inhibition by antisera prepared in rabbits against crude extracts of glycerate-grown cells; not: NaF Escherichia coli
2.7.1.31 p-hydroxymercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.31 additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.31 Ca2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
2.7.1.31 Co2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
2.7.1.31 Fe2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
2.7.1.31 Mg2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
2.7.1.31 Mn2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.31 Escherichia coli
-
 Crook's strain, ATCC 8739
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.31
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.31 additional information
-
-
 Escherichia coli
2.7.1.31 210
-
 pH 7.4 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.31 -40°C, stable for more than 2 years Escherichia coli
2.7.1.31 4°C, highly purified crystalline enzyme, 45% ethanol, stable for several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.31 ATP + (R)-glycerate
-
 Escherichia coli ADP + 3-phospho-(R)-glycerate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.31 7 7.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.31 7
-
 stable Escherichia coli
2.7.1.31 10
-
 stable below Escherichia coli