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Literature summary extracted from

  • Thorner, J.W.; Paulus, H.
    Glycerol and glycerate kinases (1973), The Enzymes,3rd ed. (Boyer,P. D. ,ed. ), 8, 487-508.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.30 glycerol affords considerable stabilization at the unfavorable pH values Mycobacterium sp.
2.7.1.30 pigeon liver enzyme is sensitive to extreme dilution but can be stabilized by addition of 0.01% bovine serum albumin Columba sp.
2.7.1.30 the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated Rattus norvegicus
2.7.1.30 the enzyme in crude extracts is stable to freezing and thawing, while more purified preparations are inactivated Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 ADP
-
 Escherichia coli
2.7.1.30 ADP
-
 Gallus gallus
2.7.1.30 ADP
-
 Rattus norvegicus
2.7.1.30 AMP
-
 Saccharomyces cerevisiae
2.7.1.30 fructose 1,6-diphosphate not Candida mycoderma
2.7.1.30 fructose 1,6-diphosphate
-
 Escherichia coli
2.7.1.30 fructose 1,6-diphosphate not Neurospora crassa
2.7.1.30 glycerol 3-phosphate
-
 Bacillus subtilis
2.7.1.30 glycerol 3-phosphate
-
 Bombus sp.
2.7.1.30 glycerol 3-phosphate
-
 Bos taurus
2.7.1.30 glycerol 3-phosphate
-
 Candida mycoderma
2.7.1.30 glycerol 3-phosphate
-
 Cavia porcellus
2.7.1.30 glycerol 3-phosphate
-
 Clostridium novyi
2.7.1.30 glycerol 3-phosphate
-
 Columba sp.
2.7.1.30 glycerol 3-phosphate
-
 Cyberlindnera jadinii
2.7.1.30 glycerol 3-phosphate
-
 Enterococcus faecalis
2.7.1.30 glycerol 3-phosphate
-
 Escherichia coli
2.7.1.30 glycerol 3-phosphate
-
 Felis catus
2.7.1.30 glycerol 3-phosphate
-
 Geotrichum candidum
2.7.1.30 glycerol 3-phosphate
-
 Gluconobacter oxydans
2.7.1.30 glycerol 3-phosphate
-
 Halobacterium salinarum
2.7.1.30 glycerol 3-phosphate
-
 Homo sapiens
2.7.1.30 glycerol 3-phosphate
-
 Klebsiella aerogenes
2.7.1.30 glycerol 3-phosphate
-
 Locusta sp.
2.7.1.30 glycerol 3-phosphate
-
 Mesocricetus auratus
2.7.1.30 glycerol 3-phosphate
-
 Mus musculus
2.7.1.30 glycerol 3-phosphate
-
 Mycobacterium butyricum
2.7.1.30 glycerol 3-phosphate
-
 Mycobacterium sp.
2.7.1.30 glycerol 3-phosphate
-
 Mycobacterium tuberculosis
2.7.1.30 glycerol 3-phosphate
-
 Mycolicibacterium smegmatis
2.7.1.30 glycerol 3-phosphate
-
 Neurospora crassa
2.7.1.30 glycerol 3-phosphate
-
 Nocardia asteroides
2.7.1.30 glycerol 3-phosphate
-
 Oryctolagus cuniculus
2.7.1.30 glycerol 3-phosphate
-
 Pseudomonas aeruginosa
2.7.1.30 glycerol 3-phosphate competitive to glycerol Rattus norvegicus
2.7.1.30 glycerol 3-phosphate
-
 Saccharomyces cerevisiae
2.7.1.30 glycerol 3-phosphate
-
 Shigella sonnei
2.7.1.30 glycerol 3-phosphate
-
 Staphylococcus aureus
2.7.1.30 glycerol 3-phosphate
-
 trout
2.7.1.30 glycerol 3-phosphate
-
 Wickerhamomyces anomalus
2.7.1.30 iodoacetamide
-
 Candida mycoderma
2.7.1.30 iodoacetamide
-
 Rattus norvegicus
2.7.1.30 iodoacetate
-
 Escherichia coli
2.7.1.30 N-ethylmaleimide
-
 Escherichia coli
2.7.1.30 N-ethylmaleimide
-
 Rattus norvegicus
2.7.1.30 p-chloromercuribenzoate
-
 Candida mycoderma
2.7.1.30 p-chloromercuribenzoate
-
 Rattus norvegicus
2.7.1.30 p-Hydroxymercuriphenylsulfonate
-
 Escherichia coli
2.7.1.31 EDTA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 additional information
-
 additional information
-
 Gallus gallus
2.7.1.30 additional information
-
 additional information
-
 Mus musculus
2.7.1.30 additional information
-
 additional information
-
 Escherichia coli
2.7.1.30 additional information
-
 additional information
-
 Rattus norvegicus
2.7.1.30 additional information
-
 additional information
-
 Bos taurus
2.7.1.30 additional information
-
 additional information
-
 Columba sp.
2.7.1.30 additional information
-
 additional information
-
 Candida mycoderma
2.7.1.31 additional information
-
 additional information
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.30 cytosol described in the most tissues Cavia porcellus 5829
-
2.7.1.30 cytosol described in the most tissues Staphylococcus aureus 5829
-
2.7.1.30 cytosol described in the most tissues Bacillus subtilis 5829
-
2.7.1.30 cytosol described in the most tissues Cyberlindnera jadinii 5829
-
2.7.1.30 cytosol described in the most tissues Mus musculus 5829
-
2.7.1.30 cytosol described in the most tissues Escherichia coli 5829
-
2.7.1.30 cytosol described in the most tissues Homo sapiens 5829
-
2.7.1.30 cytosol described in the most tissues Rattus norvegicus 5829
-
2.7.1.30 cytosol described in the most tissues Saccharomyces cerevisiae 5829
-
2.7.1.30 cytosol described in the most tissues Bos taurus 5829
-
2.7.1.30 cytosol described in the most tissues Oryctolagus cuniculus 5829
-
2.7.1.30 cytosol described in the most tissues Mycolicibacterium smegmatis 5829
-
2.7.1.30 cytosol described in the most tissues Neurospora crassa 5829
-
2.7.1.30 cytosol described in the most tissues Klebsiella aerogenes 5829
-
2.7.1.30 cytosol described in the most tissues Pseudomonas aeruginosa 5829
-
2.7.1.30 cytosol described in the most tissues Halobacterium salinarum 5829
-
2.7.1.30 cytosol described in the most tissues Enterococcus faecalis 5829
-
2.7.1.30 cytosol described in the most tissues Mycobacterium tuberculosis 5829
-
2.7.1.30 cytosol described in the most tissues Mesocricetus auratus 5829
-
2.7.1.30 cytosol described in the most tissues Felis catus 5829
-
2.7.1.30 cytosol described in the most tissues Mycobacterium sp. 5829
-
2.7.1.30 cytosol described in the most tissues Nocardia asteroides 5829
-
2.7.1.30 cytosol described in the most tissues Gluconobacter oxydans 5829
-
2.7.1.30 cytosol described in the most tissues Mycobacterium butyricum 5829
-
2.7.1.30 cytosol described in the most tissues Wickerhamomyces anomalus 5829
-
2.7.1.30 cytosol described in the most tissues Shigella sonnei 5829
-
2.7.1.30 cytosol described in the most tissues trout 5829
-
2.7.1.30 cytosol described in the most tissues Geotrichum candidum 5829
-
2.7.1.30 cytosol described in the most tissues Columba sp. 5829
-
2.7.1.30 cytosol described in the most tissues Locusta sp. 5829
-
2.7.1.30 cytosol described in the most tissues Bombus sp. 5829
-
2.7.1.30 cytosol described in the most tissues Candida mycoderma 5829
-
2.7.1.30 cytosol described in the most tissues Clostridium novyi 5829
-
2.7.1.30 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.30 Mg2+ required Escherichia coli
2.7.1.31 K+ stimulation Escherichia coli
2.7.1.31 Mg2+ required Escherichia coli
2.7.1.31 additional information divalent cation required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 60000
-
 4 * 60000, SDS-PAGE Saccharomyces cerevisiae
2.7.1.30 140000
-
 gel filtration, zone sedimentation in sucrose density gradient Neurospora crassa
2.7.1.30 210000 217000 equilibrium sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.30 ATP + glycerol Escherichia coli enzyme functions primarily in the utilization of glycerol as a carbon and energy source ?
-
 ?
2.7.1.30 ATP + glycerol Mesocricetus auratus higher organisms: salvage of glycerol released upon lipolysis ?
-
 ?
2.7.1.31 ATP + glycerate Escherichia coli
-
 ADP + 3-phospho-(R)-glycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Bacillus subtilis
-
-
-
2.7.1.30 Bombus sp.
-
 queen bumblebee
-
2.7.1.30 Bos taurus
-
-
-
2.7.1.30 Candida mycoderma
-
-
-
2.7.1.30 Cavia porcellus
-
-
-
2.7.1.30 Clostridium novyi
-
-
-
2.7.1.30 Columba sp.
-
 pigeon
-
2.7.1.30 Cyberlindnera jadinii
-
-
-
2.7.1.30 Enterococcus faecalis
-
-
-
2.7.1.30 Escherichia coli
-
-
-
2.7.1.30 Felis catus
-
-
-
2.7.1.30 Gallus gallus
-
-
-
2.7.1.30 Geotrichum candidum
-
-
-
2.7.1.30 Gluconobacter oxydans
-
-
-
2.7.1.30 Halobacterium salinarum
-
-
-
2.7.1.30 Homo sapiens
-
-
-
2.7.1.30 Klebsiella aerogenes
-
-
-
2.7.1.30 Locusta sp.
-
-
-
2.7.1.30 Mesocricetus auratus
-
-
-
2.7.1.30 Mus musculus
-
-
-
2.7.1.30 Mycobacterium butyricum
-
-
-
2.7.1.30 Mycobacterium sp.
-
 607
-
2.7.1.30 Mycobacterium sp. 607
-
 607
-
2.7.1.30 Mycobacterium tuberculosis
-
-
-
2.7.1.30 Mycolicibacterium smegmatis
-
-
-
2.7.1.30 Neurospora crassa
-
-
-
2.7.1.30 Nocardia asteroides
-
-
-
2.7.1.30 Oryctolagus cuniculus
-
-
-
2.7.1.30 Pseudomonas aeruginosa
-
-
-
2.7.1.30 Rattus norvegicus
-
-
-
2.7.1.30 Saccharomyces cerevisiae
-
-
-
2.7.1.30 Shigella sonnei
-
-
-
2.7.1.30 Staphylococcus aureus
-
-
-
2.7.1.30 trout
-
-
-
2.7.1.30 Wickerhamomyces anomalus
-
-
-
2.7.1.31 Escherichia coli
-
 Crook's strain, ATCC 8739
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.1.30 susceptible to inactivation by oxidation of sulfhydryl groups Columba sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.30 ATP + glycerol = ADP + sn-glycerol 3-phosphate ordered mechanism Escherichia coli
a
2.7.1.30 ATP + glycerol = ADP + sn-glycerol 3-phosphate ordered mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.30 adipose tissue
-
 Gallus gallus
-
2.7.1.30 adipose tissue
-
 Bos taurus
-
2.7.1.30 adipose tissue white and brown Mus musculus
-
2.7.1.30 adipose tissue white and brown Homo sapiens
-
2.7.1.30 adipose tissue white and brown Rattus norvegicus
-
2.7.1.30 adipose tissue white and brown Oryctolagus cuniculus
-
2.7.1.30 adipose tissue white and brown Locusta sp.
-
2.7.1.30 intestine
-
 Rattus norvegicus
-
2.7.1.30 intestine
-
 Mesocricetus auratus
-
2.7.1.30 intestine
-
 Felis catus
-
2.7.1.30 kidney
-
 Rattus norvegicus
-
2.7.1.30 liver
-
 Gallus gallus
-
2.7.1.30 liver
-
 Mus musculus
-
2.7.1.30 liver
-
 Rattus norvegicus
-
2.7.1.30 liver
-
 Bos taurus
-
2.7.1.30 liver
-
 Mesocricetus auratus
-
2.7.1.30 liver
-
 trout
-
2.7.1.30 liver
-
 Columba sp.
-
2.7.1.30 additional information enzyme activity in various tissues Cavia porcellus
-
2.7.1.30 additional information enzyme activity in various tissues Staphylococcus aureus
-
2.7.1.30 additional information enzyme activity in various tissues Bacillus subtilis
-
2.7.1.30 additional information enzyme activity in various tissues Cyberlindnera jadinii
-
2.7.1.30 additional information enzyme activity in various tissues Mus musculus
-
2.7.1.30 additional information enzyme activity in various tissues Escherichia coli
-
2.7.1.30 additional information enzyme activity in various tissues Homo sapiens
-
2.7.1.30 additional information enzyme activity in various tissues Rattus norvegicus
-
2.7.1.30 additional information enzyme activity in various tissues Saccharomyces cerevisiae
-
2.7.1.30 additional information enzyme activity in various tissues Bos taurus
-
2.7.1.30 additional information enzyme activity in various tissues Oryctolagus cuniculus
-
2.7.1.30 additional information enzyme activity in various tissues Mycolicibacterium smegmatis
-
2.7.1.30 additional information enzyme activity in various tissues Neurospora crassa
-
2.7.1.30 additional information enzyme activity in various tissues Klebsiella aerogenes
-
2.7.1.30 additional information enzyme activity in various tissues Pseudomonas aeruginosa
-
2.7.1.30 additional information enzyme activity in various tissues Halobacterium salinarum
-
2.7.1.30 additional information enzyme activity in various tissues Enterococcus faecalis
-
2.7.1.30 additional information enzyme activity in various tissues Mycobacterium tuberculosis
-
2.7.1.30 additional information enzyme activity in various tissues Mesocricetus auratus
-
2.7.1.30 additional information enzyme activity in various tissues Felis catus
-
2.7.1.30 additional information enzyme activity in various tissues Mycobacterium sp.
-
2.7.1.30 additional information enzyme activity in various tissues Nocardia asteroides
-
2.7.1.30 additional information enzyme activity in various tissues Gluconobacter oxydans
-
2.7.1.30 additional information enzyme activity in various tissues Mycobacterium butyricum
-
2.7.1.30 additional information enzyme activity in various tissues Wickerhamomyces anomalus
-
2.7.1.30 additional information enzyme activity in various tissues Shigella sonnei
-
2.7.1.30 additional information enzyme activity in various tissues trout
-
2.7.1.30 additional information enzyme activity in various tissues Geotrichum candidum
-
2.7.1.30 additional information enzyme activity in various tissues Columba sp.
-
2.7.1.30 additional information enzyme activity in various tissues Locusta sp.
-
2.7.1.30 additional information enzyme activity in various tissues Bombus sp.
-
2.7.1.30 additional information enzyme activity in various tissues Candida mycoderma
-
2.7.1.30 additional information enzyme activity in various tissues Clostridium novyi
-
2.7.1.30 muscle
-
 Gallus gallus
-
2.7.1.30 muscle
-
 Rattus norvegicus
-
2.7.1.30 muscle
-
 Oryctolagus cuniculus
-
2.7.1.30 muscle
-
 trout
-
2.7.1.30 muscle
-
 Columba sp.
-
2.7.1.30 muscle
-
 Locusta sp.
-
2.7.1.30 muscle
-
 Bombus sp.
-
2.7.1.30 spermatozoon
-
 Gallus gallus
-
2.7.1.30 spermatozoon
-
 Homo sapiens
-
2.7.1.30 spermatozoon
-
 Bos taurus
-
2.7.1.30 spermatozoon
-
 Oryctolagus cuniculus
-
2.7.1.30 spermatozoon
-
 trout
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.30 -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months Rattus norvegicus
2.7.1.30 -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months Mesocricetus auratus
2.7.1.30 -20°C, pH 5.0, partially purified enzymes have half-lives of several weeks to months Columba sp.
2.7.1.30 as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years Escherichia coli
2.7.1.30 as crystalline suspension in saturated ammonium sulfate, solutions containing 10 mM glycerol, 1 mM EDTA and a thiol e.g. 2-mercaptoethanol, yeast enzyme stable for several months, E. coli enzyme for several years Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ATP + D-glyceraldehyde
-
 Escherichia coli ADP + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Escherichia coli ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + dihydroxyacetone
-
 Candida mycoderma ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.30 ATP + glycerol
-
 Gallus gallus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Cavia porcellus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Staphylococcus aureus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Bacillus subtilis ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Cyberlindnera jadinii ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mus musculus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Escherichia coli ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Homo sapiens ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Rattus norvegicus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Saccharomyces cerevisiae ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Bos taurus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Oryctolagus cuniculus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mycolicibacterium smegmatis ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Neurospora crassa ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Klebsiella aerogenes ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Pseudomonas aeruginosa ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Halobacterium salinarum ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Enterococcus faecalis ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mycobacterium tuberculosis ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mesocricetus auratus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Felis catus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mycobacterium sp. ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Nocardia asteroides ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Gluconobacter oxydans ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mycobacterium butyricum ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Wickerhamomyces anomalus ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Shigella sonnei ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor trout ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Geotrichum candidum ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Columba sp. ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Locusta sp. ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Bombus sp. ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Candida mycoderma ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Clostridium novyi ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme can utilize only ATP as phosphoryl group donor Mycobacterium sp. 607 ADP + sn-glycerol 3-phosphate
-
 ?
2.7.1.30 ATP + glycerol enzyme functions primarily in the utilization of glycerol as a carbon and energy source Escherichia coli ?
-
 ?
2.7.1.30 ATP + glycerol higher organisms: salvage of glycerol released upon lipolysis Mesocricetus auratus ?
-
 ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Escherichia coli ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 ATP + L-glyceraldehyde
-
 Candida mycoderma ADP + L-glyceraldehyde 3-phosphate
-
 ?
2.7.1.30 CTP + glycerol
-
 Gallus gallus CDP + glycerol 3-phosphate
-
 ?
2.7.1.30 CTP + glycerol
-
 Candida mycoderma CDP + glycerol 3-phosphate
-
 ?
2.7.1.30 ITP + glycerol
-
 Gallus gallus IDP + glycerol 3-phosphate
-
 ?
2.7.1.30 ITP + glycerol
-
 Candida mycoderma IDP + glycerol 3-phosphate
-
 ?
2.7.1.30 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.1.30 additional information
-
 Candida mycoderma ?
-
 ?
2.7.1.30 additional information phosphate rather than D-glyceraldehyde 3-phosphate is formed, the hydrated form of this triose is phosphorylated in position 1 to yield an unstable intermediate that decomposes to D-glyceraldehyde + phosphate Escherichia coli ?
-
 ?
2.7.1.30 UTP + glycerol
-
 Gallus gallus UDP + glycerol 3-phosphate
-
 ?
2.7.1.31 ATP + (R)-glycerate
-
 Escherichia coli ADP + 3-phospho-(R)-glycerate
-
 ?
2.7.1.31 ATP + glycerate
-
 Escherichia coli ADP + 3-phospho-(R)-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.30 tetramer 4 * 60000, SDS-PAGE Saccharomyces cerevisiae
2.7.1.30 tetramer 4 * 55000-57000, equilibrium ultracentrifugation in presence of 6 M guanidine HCl, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.30 additional information
-
-
 Escherichia coli
2.7.1.30 additional information
-
-
 Rattus norvegicus
2.7.1.30 additional information
-
 enzyme is quite heat labile Mycobacterium sp.
2.7.1.30 50
-
 5 min, 30% loss of activity Neurospora crassa
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Cavia porcellus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Staphylococcus aureus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Bacillus subtilis
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Cyberlindnera jadinii
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mus musculus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Escherichia coli
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Homo sapiens
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Rattus norvegicus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Saccharomyces cerevisiae
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Bos taurus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Oryctolagus cuniculus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mycolicibacterium smegmatis
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Neurospora crassa
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Klebsiella aerogenes
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Pseudomonas aeruginosa
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Halobacterium salinarum
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Enterococcus faecalis
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mycobacterium tuberculosis
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mesocricetus auratus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Felis catus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mycobacterium sp.
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Nocardia asteroides
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Gluconobacter oxydans
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Mycobacterium butyricum
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Wickerhamomyces anomalus
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Shigella sonnei
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods trout
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Geotrichum candidum
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Columba sp.
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Locusta sp.
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Bombus sp.
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Candida mycoderma
2.7.1.30 60
-
 in presence of 10 mM glycerol and 1 mM EDTA almost all glycerol kinases can be heated for prolonged periods Clostridium novyi
2.7.1.30 70
-
-
 Cavia porcellus
2.7.1.30 70
-
-
 Staphylococcus aureus
2.7.1.30 70
-
-
 Bacillus subtilis
2.7.1.30 70
-
-
 Cyberlindnera jadinii
2.7.1.30 70
-
-
 Mus musculus
2.7.1.30 70
-
-
 Homo sapiens
2.7.1.30 70
-
-
 Rattus norvegicus
2.7.1.30 70
-
-
 Saccharomyces cerevisiae
2.7.1.30 70
-
-
 Bos taurus
2.7.1.30 70
-
-
 Oryctolagus cuniculus
2.7.1.30 70
-
-
 Mycolicibacterium smegmatis
2.7.1.30 70
-
-
 Neurospora crassa
2.7.1.30 70
-
-
 Klebsiella aerogenes
2.7.1.30 70
-
-
 Pseudomonas aeruginosa
2.7.1.30 70
-
-
 Halobacterium salinarum
2.7.1.30 70
-
-
 Enterococcus faecalis
2.7.1.30 70
-
-
 Mycobacterium tuberculosis
2.7.1.30 70
-
-
 Mesocricetus auratus
2.7.1.30 70
-
-
 Felis catus
2.7.1.30 70
-
-
 Mycobacterium sp.
2.7.1.30 70
-
-
 Nocardia asteroides
2.7.1.30 70
-
-
 Gluconobacter oxydans
2.7.1.30 70
-
-
 Mycobacterium butyricum
2.7.1.30 70
-
-
 Wickerhamomyces anomalus
2.7.1.30 70
-
-
 Shigella sonnei
2.7.1.30 70
-
-
 trout
2.7.1.30 70
-
-
 Geotrichum candidum
2.7.1.30 70
-
-
 Columba sp.
2.7.1.30 70
-
-
 Locusta sp.
2.7.1.30 70
-
-
 Bombus sp.
2.7.1.30 70
-
-
 Candida mycoderma
2.7.1.30 70
-
-
 Clostridium novyi
2.7.1.30 70
-
 t1/2: 5 min Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.31 350
-
 D-glycerate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.30 7 7.5
-
 Mycobacterium sp.
2.7.1.30 7 7.5
-
 Clostridium novyi
2.7.1.30 8
-
-
 Saccharomyces cerevisiae
2.7.1.31 7 7.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.30 additional information
-
 glycerol affords considerable stabilization at the unfavorable pH values, glycerol kinases from microorganisms most stable at a neutral pH, glycerol kinases from higher organisms most stable in an acidic pH range Rattus norvegicus
2.7.1.30 5
-
 0°C, 24 h, complete loss of activity, 30°C, 30 min, 75% loss of activity Escherichia coli
2.7.1.30 5
-
 0°C, 6 h, without glycerol, completely stable Columba sp.
2.7.1.30 6 9 30°C, in presence of glycerol, completely stable for 30 min Mycobacterium sp.
2.7.1.30 7
-
 0°C, 6 h, without glycerol, 90% loss of activity Columba sp.
2.7.1.30 7
-
 0°C, 24 h, 50% loss of activity Escherichia coli
2.7.1.30 7.5
-
 25°C, no loss of activity after several h Rattus norvegicus
2.7.1.30 9.8
-
 25°C, half-life: 6.5 min Rattus norvegicus