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Literature summary extracted from

  • Schwenn, J.D.; Jender, H.G.
    Purification and properties of the ATP:adenylylsulfate 3'-phosphotransferase from chlamydomonas reinhardii (1984), Arch. Microbiol., 138, 9-14.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 dithioerythritol 2 mM, 200% activation Chlamydomonas reinhardtii
2.7.1.25 dithiothreitol activation Chlamydomonas reinhardtii
2.7.1.25 dithiothreitol 2 mM, 200% activation Chlamydomonas reinhardtii
2.7.1.25 additional information not activated by 2-mercaptoethanol Chlamydomonas reinhardtii
2.7.1.25 Na2SO3 5 mM, 200% activation Chlamydomonas reinhardtii

General Stability

EC Number General Stability Organism
2.7.1.25 more stable in ampholyte polybuffer 74 than in Tris or imidazole buffers Chlamydomonas reinhardtii
2.7.1.25 slow freezing can cause loss of activity Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 4-Aminophenylacetate 0.008 mM, 50% inhibition Chlamydomonas reinhardtii
2.7.1.25 AMP
-
Chlamydomonas reinhardtii
2.7.1.25 Bromosuccinimide strong inhibition Chlamydomonas reinhardtii
2.7.1.25 CMP
-
Chlamydomonas reinhardtii
2.7.1.25 Co2+ 2 mM, 63% inhibition Chlamydomonas reinhardtii
2.7.1.25 Cu2+ 2 mM, 90% inhibition Chlamydomonas reinhardtii
2.7.1.25 GMP
-
Chlamydomonas reinhardtii
2.7.1.25 N-ethylmaleimide 0.02 mM, 50% inhibition Chlamydomonas reinhardtii
2.7.1.25 p-chloromercuribenzoate 0.005 mM, 50% inhibition Chlamydomonas reinhardtii
2.7.1.25 UMP
-
Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.002
-
adenosine 5'-phosphosulfate pH 6.8, 30°C Chlamydomonas reinhardtii
2.7.1.25 0.007
-
ATP pH 6.8, 30°C Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 K+ 10 mM, 60% stimulation compared to Mg2+ Chlamydomonas reinhardtii
2.7.1.25 Mg2+ activation Chlamydomonas reinhardtii
2.7.1.25 Mn2+
-
Chlamydomonas reinhardtii
2.7.1.25 additional information not activated by Cu2+ or Na+ Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 44000
-
x * 44000, SDS-PAGE Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Chlamydomonas reinhardtii
-
CW15, cell wall mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 0.136
-
-
Chlamydomonas reinhardtii

Storage Stability

EC Number Storage Stability Organism
2.7.1.25 -20°C, stable to prolonged storage Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate ATP can be replaced by GTP, ITP or UTP Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.1.25 ATP + adenosine 5-phosphosulfate GTP, ITP or UTP are poor substrates Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.1.25 CTP + adenosine 5-phosphosulfate
-
Chlamydomonas reinhardtii CDP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
2.7.1.25 dATP + adenosine 5-phosphosulfate
-
Chlamydomonas reinhardtii dADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 ? x * 44000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.25 30
-
assay at Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.25 additional information
-
pI: 6.2 Chlamydomonas reinhardtii
2.7.1.25 6
-
-
Chlamydomonas reinhardtii
2.7.1.25 6.5 7.5 in MES-Tricine-HEPES buffer Chlamydomonas reinhardtii
2.7.1.25 6.8
-
in imidazole buffer Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.25 5.5 8.5 approx. 80% of maximal activity at pH 5.5, approx. half-maximal activity at pH 8.5 Chlamydomonas reinhardtii
2.7.1.25 6 8 approx. 80% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0, imidazole buffer Chlamydomonas reinhardtii
2.7.1.25 6 8.5 approx. 70% of maximal activity at pH 6.0, approx. 75% of maximal activity at pH 8.5, MES-Tricine-HEPES buffer Chlamydomonas reinhardtii