EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | dithioerythritol | 2 mM, 200% activation | Chlamydomonas reinhardtii | |
2.7.1.25 | dithiothreitol | activation | Chlamydomonas reinhardtii | |
2.7.1.25 | dithiothreitol | 2 mM, 200% activation | Chlamydomonas reinhardtii | |
2.7.1.25 | additional information | not activated by 2-mercaptoethanol | Chlamydomonas reinhardtii | |
2.7.1.25 | Na2SO3 | 5 mM, 200% activation | Chlamydomonas reinhardtii |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.25 | more stable in ampholyte polybuffer 74 than in Tris or imidazole buffers | Chlamydomonas reinhardtii |
2.7.1.25 | slow freezing can cause loss of activity | Chlamydomonas reinhardtii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | 4-Aminophenylacetate | 0.008 mM, 50% inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | AMP | - |
Chlamydomonas reinhardtii | |
2.7.1.25 | Bromosuccinimide | strong inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | CMP | - |
Chlamydomonas reinhardtii | |
2.7.1.25 | Co2+ | 2 mM, 63% inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | Cu2+ | 2 mM, 90% inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | GMP | - |
Chlamydomonas reinhardtii | |
2.7.1.25 | N-ethylmaleimide | 0.02 mM, 50% inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | p-chloromercuribenzoate | 0.005 mM, 50% inhibition | Chlamydomonas reinhardtii | |
2.7.1.25 | UMP | - |
Chlamydomonas reinhardtii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.25 | 0.002 | - |
adenosine 5'-phosphosulfate | pH 6.8, 30°C | Chlamydomonas reinhardtii | |
2.7.1.25 | 0.007 | - |
ATP | pH 6.8, 30°C | Chlamydomonas reinhardtii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | K+ | 10 mM, 60% stimulation compared to Mg2+ | Chlamydomonas reinhardtii | |
2.7.1.25 | Mg2+ | activation | Chlamydomonas reinhardtii | |
2.7.1.25 | Mn2+ | - |
Chlamydomonas reinhardtii | |
2.7.1.25 | additional information | not activated by Cu2+ or Na+ | Chlamydomonas reinhardtii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 44000 | - |
x * 44000, SDS-PAGE | Chlamydomonas reinhardtii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.25 | Chlamydomonas reinhardtii | - |
CW15, cell wall mutant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.25 | ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose | Chlamydomonas reinhardtii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 0.136 | - |
- |
Chlamydomonas reinhardtii |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.25 | -20°C, stable to prolonged storage | Chlamydomonas reinhardtii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.25 | ATP + adenosine 5-phosphosulfate | ATP can be replaced by GTP, ITP or UTP | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
2.7.1.25 | ATP + adenosine 5-phosphosulfate | GTP, ITP or UTP are poor substrates | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Chlamydomonas reinhardtii | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | r | |
2.7.1.25 | CTP + adenosine 5-phosphosulfate | - |
Chlamydomonas reinhardtii | CDP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
2.7.1.25 | dATP + adenosine 5-phosphosulfate | - |
Chlamydomonas reinhardtii | dADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.25 | ? | x * 44000, SDS-PAGE | Chlamydomonas reinhardtii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 30 | - |
assay at | Chlamydomonas reinhardtii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | additional information | - |
pI: 6.2 | Chlamydomonas reinhardtii |
2.7.1.25 | 6 | - |
- |
Chlamydomonas reinhardtii |
2.7.1.25 | 6.5 | 7.5 | in MES-Tricine-HEPES buffer | Chlamydomonas reinhardtii |
2.7.1.25 | 6.8 | - |
in imidazole buffer | Chlamydomonas reinhardtii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 5.5 | 8.5 | approx. 80% of maximal activity at pH 5.5, approx. half-maximal activity at pH 8.5 | Chlamydomonas reinhardtii |
2.7.1.25 | 6 | 8 | approx. 80% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0, imidazole buffer | Chlamydomonas reinhardtii |
2.7.1.25 | 6 | 8.5 | approx. 70% of maximal activity at pH 6.0, approx. 75% of maximal activity at pH 8.5, MES-Tricine-HEPES buffer | Chlamydomonas reinhardtii |