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Literature summary extracted from

  • Schwenn, J.D.; Jender, H.G.
    A kinetic investigation of the APS-kinase from chlamydomonas reinhardii CW 15 (1981), Phytochemistry, 20, 601-604.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate substrate inhibition above 0.03-0.04 mM Chlamydomonas reinhardtii
2.7.1.25 ATP in the absence of ATP regenerating system, substrate inhibition above 0.6 mM, in the presence of ATP regenerating system, substrate inhibition above 0.2 mM Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.006
-
 adenosine 5'-phosphosulfate pH 8.0 Chlamydomonas reinhardtii
2.7.1.25 0.05 0.06 ATP pH 8.0 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ activation Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Chlamydomonas reinhardtii
-
 CW15, cell wall mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 ammonium sulfate, Sephacryl S-200, partially purified Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 0.002 0.006
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Chlamydomonas reinhardtii ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r