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Literature summary extracted from

  • Kanno, N.; Sato, M.; Sato, Y.
    Purification and properties of adenosine-5'-phosphosulfate kinase from the marine red macroalga Porphyra yezoensis Ueda (1990), Bot. Mar., 33, 369-374.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate
-
 Neopyropia yezoensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.00045
-
 adenosine 5'-phosphosulfate
-
 Neopyropia yezoensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 additional information a divalent cation is required for activity Neopyropia yezoensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 31000
-
 2 * 31000, dissociation Neopyropia yezoensis
2.7.1.25 61000
-
 gel filtration Neopyropia yezoensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Neopyropia yezoensis
-
 Ueda, marine alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 ammonium sulfate, affinity chromatography, gel filtration, ion-exchange chromatography Neopyropia yezoensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 thallus
-
 Neopyropia yezoensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate ATP can be replaced by GTP, ITP or UTP Neopyropia yezoensis ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate optimal concentration of APS at 0.003 mM Neopyropia yezoensis ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Neopyropia yezoensis ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?
2.7.1.25 CTP + adenosine 5-phosphosulfate
-
 Neopyropia yezoensis CDP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 31000, dissociation Neopyropia yezoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.25 7
-
-
 Neopyropia yezoensis