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Literature summary extracted from

  • Wang, L.; Saada, A.; Eriksson, S.
    Kinetic properties of mutant human thymidine kinase 2 suggest a mechanism for mitochondrial DNA depletion myopathy (2003), J. Biol. Chem., 278, 6963-6968.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 TK2 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.21 H121N similar subunit structure to wild-type enzyme, decreased enzyme activity Homo sapiens
2.7.1.21 I212N similar subunit structure to wild-type enzyme, less than 1% activity compared to wild-type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dCTP
-
 Homo sapiens
2.7.1.21 deoxcytidine
-
 Homo sapiens
2.7.1.21 dTTP
-
 Homo sapiens
2.7.1.21 thymidine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0057
-
 thymidine H121N mutant, pH 7.6 Homo sapiens
2.7.1.21 0.011
-
 deoxycytidine wild-type and H121N mutant, pH 7.6 Homo sapiens
2.7.1.21 0.013
-
 thymidine wild-type, pH 7.6 Homo sapiens
2.7.1.21 0.018
-
 thymidine I212 mutant, pH 7.6 Homo sapiens
2.7.1.21 0.025
-
 ATP wild-type, thymidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.037
-
 ATP wild-type, deoxycytidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.059
-
 ATP I212 mutant, deoxycytidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.08
-
 ATP H121N mutant, thymidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.095
-
 ATP I212 mutant, thymidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.162
-
 ATP H121N mutant, deoxycytidine as substrate, pH 7.6 Homo sapiens
2.7.1.21 0.568
-
 deoxycytidine I212 mutant, pH 7.6 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens O00142
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.00036
-
 dCTP H121N mutant, pH 7.6, competitive with thymidine as substrate Homo sapiens
2.7.1.21 0.00079
-
 dCTP wild-type enzyme, pH 7.6, competitive with thymidine as substrate Homo sapiens
2.7.1.21 0.00087
-
 dCTP wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
2.7.1.21 0.00089
-
 dTTP H121N mutant, pH 7.6, competitive with thymidine as substrate Homo sapiens
2.7.1.21 0.00089
-
 dCTP H121N mutant, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
2.7.1.21 0.002
-
 dTTP wild-type enzyme, pH 7.6, competitive with thymidine as substrate Homo sapiens
2.7.1.21 0.0025
-
 dTTP wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate Homo sapiens
2.7.1.21 0.0036
-
 thymidine H121N mutant, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
2.7.1.21 0.004
-
 deoxycytidine H121N mutant, pH 7.6, non-competitive with thymidine as substrate Homo sapiens
2.7.1.21 0.0049
-
 thymidine wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate Homo sapiens
2.7.1.21 0.04
-
 deoxycytidine wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate Homo sapiens