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Literature summary extracted from

  • Cheng, Y.C.; Prusoff, W.H.
    Mouse ascites Sarcoma 180 deoxythymidine kinase. General properties and inhibition studies (1974), Biochemistry, 13, 1179-1185.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 stability decreases with increasing purity, ATP, thymidine, Mg2+ protect Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 2'-deoxy-3'-aminothymidine 5 mM Mus musculus
2.7.1.21 2-mercaptoethanol 5 mM Mus musculus
2.7.1.21 5'-aminothymidine
-
 Mus musculus
2.7.1.21 dTTP
-
 Mus musculus
2.7.1.21 additional information 5'-derivatives of thymidine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.009
-
 thymidine pH 7.8, 37°C Mus musculus
2.7.1.21 0.15
-
 CTP ATP Mus musculus
2.7.1.21 0.15
-
 ATP pH 7.8, 37°C Mus musculus
2.7.1.21 0.3
-
 ATP GTP Mus musculus
2.7.1.21 0.3
-
 GTP pH 7.8, 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 sarcoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 0.053
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.21 -20°C, 0.1 mM thymidine, 1 week, 30% loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine ATP can be substituted by GTP Mus musculus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dGTP Mus musculus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by ATP-arabinoside Mus musculus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by UTP Mus musculus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dCTP Mus musculus ADP + dTMP
-
 ?
2.7.1.21 CTP + thymidine
-
 Mus musculus CDP + thymidine 5'-phosphate
-
 ?
2.7.1.21 GTP + thymidine
-
 Mus musculus GDP + thymidine 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.21 additional information
-
 ATP, thymidine, Mg2+ protect against thermal inactivation Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 additional information
-
 additional information
-
 Mus musculus
2.7.1.21 0.003
-
 2'-deoxy-3'-aminothymidine pH 7.8, 37°C Mus musculus
2.7.1.21 3
-
 5'-aminothymidine pH 7.8, 37°C Mus musculus