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Literature summary extracted from

  • Waldman, A.S.; Haeusslein, E.; Milman, G.
    Purification and characterization of herpes simplex virus (type 1) thymidine kinase produced in Escherichia coli by a high efficiency expression plasmid utilizing a lambda PL promoter and cI857 temperature-sensitive repressor (1983), J. Biol. Chem., 258, 11571-11575.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21
-
 Herpes simplex virus

General Stability

EC Number General Stability Organism
2.7.1.21 2-mercaptoethanol protects against thermal inactivation Herpes simplex virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 acyclovir syn. 9-[(2-hydroxyethoxy)methyl]guanine Herpes simplex virus
2.7.1.21 iododeoxycytidine
-
 Herpes simplex virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0006
-
 thymidine
-
 Herpes simplex virus
2.7.1.21 0.118
-
 ATP
-
 Herpes simplex virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 42000
-
 2 * 42000, SDS-PAGE Herpes simplex virus
2.7.1.21 70000 80000 gel filtration Herpes simplex virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Herpes simplex virus
-
 type 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 expressed in Escherichia coli Herpes simplex virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 0.13
-
-
 Herpes simplex virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine ATP can be substituted by GTP Herpes simplex virus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dGTP Herpes simplex virus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dCTP Herpes simplex virus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dATP Herpes simplex virus ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 42000, SDS-PAGE Herpes simplex virus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.21 additional information
-
 2-mercaptoethanol protects against thermal inactivation Herpes simplex virus
2.7.1.21 40
-
 the half-life of 3 min increases to 6 min in presence of 2 mM ATP or to 30 min in presence of 0.12 mM thymidine Herpes simplex virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 8.5
-
-
 Herpes simplex virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.21 6 10 enzyme is active between pH 6 and pH 10 Herpes simplex virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.014
-
 iododeoxycytidine pH 7.8, 37°C Herpes simplex virus
2.7.1.21 0.3
-
 acyclovir pH 7.8, 37°C Herpes simplex virus