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Literature summary extracted from

  • Bresnick, E.
    Deoxythymidine kinase in regenerating rat liver (1978), Methods Enzymol., 51, 360-365.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 5-bromodeoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 5-Chlorodeoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 5-fluorodeoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 5-iododeoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 5-Trifluoromethyldeoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 Ba2+
-
 Rattus norvegicus
2.7.1.21 Be2+
-
 Rattus norvegicus
2.7.1.21 Ca2+
-
 Rattus norvegicus
2.7.1.21 Co2+
-
 Rattus norvegicus
2.7.1.21 deoxyuridine competitive to thymidine Rattus norvegicus
2.7.1.21 dTTP non-competitive Rattus norvegicus
2.7.1.21 Fe2+
-
 Rattus norvegicus
2.7.1.21 Mn2+
-
 Rattus norvegicus
2.7.1.21 Ni2+
-
 Rattus norvegicus
2.7.1.21 Sr2+
-
 Rattus norvegicus
2.7.1.21 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0056
-
 thymidine pH 8.0, 37°C, Km is markedly dependent on pH Rattus norvegicus
2.7.1.21 0.8
-
 MgCl2 pH 8 Rattus norvegicus
2.7.1.21 2.6
-
 ATP at concentrations above 1 mM, pH 8, 37°C Rattus norvegicus
2.7.1.21 20
-
 ATP at concentrations below 1 mM, pH 8, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Ca2+ can partially substitute for Mg2+ Rattus norvegicus
2.7.1.21 Co2+ can partially substitute for Mg2+ Rattus norvegicus
2.7.1.21 Mg2+ Km: 0.8 mM Rattus norvegicus
2.7.1.21 Mn2+ can partially substitute for Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 81000
-
 sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 0.06
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxyuridine
-
 Rattus norvegicus ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-bromo-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-chloro-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-fluoro-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-iodo-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-trifluoromethyl-2'-deoxyuridine
-
 Rattus norvegicus ADP + 5-trifluoromethyl-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 6-aza-2'-deoxythymidine
-
 Rattus norvegicus ADP + 6-aza-2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + thymidine
-
 Rattus norvegicus ADP + dTMP
-
 ?
2.7.1.21 additional information 5-halogenated cytidines, bromouridine, iodouridine, uridine, 5-fluorodeoxycytidine, purine ribonucleosides Rattus norvegicus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 8
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.001
-
 Be2+ pH 8, 37°C Rattus norvegicus
2.7.1.21 0.022
-
 5-fluorodeoxyuridine pH 8, 37°C Rattus norvegicus
2.7.1.21 0.11
-
 deoxyuridine pH 8, 37°C Rattus norvegicus