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Literature summary extracted from

  • Faye, F.; Le Floc'h, F.
    Adenosine kinase of peach tree flower buds: purification and properties (1997), Plant Physiol. Biochem., 35, 15-22.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 2'-deoxyadenosine no inhibition Prunus persica
2.7.1.20 3'-deoxyadenosine no inhibition Prunus persica
2.7.1.20 ADP non competitive with respect to adenosine and competitive with respect to ATP; product inhibition Prunus persica
2.7.1.20 AMP mixed-type inhibition towards adenosine and ATP; product inhibition Prunus persica
2.7.1.20 GDP non competitive with respect to adenosine and competitive with respect to ATP Prunus persica
2.7.1.20 N6-delta2-isopentenyl-adenosine competitive with respect to adenosine Prunus persica
2.7.1.20 NH4+ slight inhibition Prunus persica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.0018 0.006 adenosine pH 9, 25ºC Prunus persica
2.7.1.20 0.2 0.7 ATP pH 9, 25ºC Prunus persica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 KCl strong activation, maximum activity at 100 mM Prunus persica
2.7.1.20 Mg2+ 0.3-0.4 mM, in the presence of K+ Prunus persica
2.7.1.20 Mn2+ at 0.4-0.5 mM, in the presence of K+ Prunus persica
2.7.1.20 Rb+ activation, at 100 mM, weaker than K+ Prunus persica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 45000
-
gel filtration Prunus persica
2.7.1.20 434000
-
1 * 434000, SDS-PAGE Prunus persica

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Prunus persica
-
Batsch cv Redhaven, peach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 ammonium sulfate fractionation, gel filtration ion exchange and affinity chromatography Prunus persica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 flower buds Prunus persica
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -25ºC, variable losses of activity Prunus persica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine best substrates Prunus persica ADP + AMP
-
?
2.7.1.20 CTP + adenosine
-
Prunus persica CDP + AMP
-
?
2.7.1.20 GTP + adenosine less efficient than ATP Prunus persica GDP + AMP
-
?
2.7.1.20 UTP + adenosine
-
Prunus persica UDP + AMP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 monomer 1 * 434000, SDS-PAGE Prunus persica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 8.5 9.5
-
Prunus persica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.20 8.5 9.5 sharp drop of activity outside the range, especially between pH 6.5 and pH 8.5 Prunus persica