EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | 2'-deoxyadenosine | no inhibition | Prunus persica | |
2.7.1.20 | 3'-deoxyadenosine | no inhibition | Prunus persica | |
2.7.1.20 | ADP | non competitive with respect to adenosine and competitive with respect to ATP; product inhibition | Prunus persica | |
2.7.1.20 | AMP | mixed-type inhibition towards adenosine and ATP; product inhibition | Prunus persica | |
2.7.1.20 | GDP | non competitive with respect to adenosine and competitive with respect to ATP | Prunus persica | |
2.7.1.20 | N6-delta2-isopentenyl-adenosine | competitive with respect to adenosine | Prunus persica | |
2.7.1.20 | NH4+ | slight inhibition | Prunus persica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.20 | 0.0018 | 0.006 | adenosine | pH 9, 25ºC | Prunus persica | |
2.7.1.20 | 0.2 | 0.7 | ATP | pH 9, 25ºC | Prunus persica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | KCl | strong activation, maximum activity at 100 mM | Prunus persica | |
2.7.1.20 | Mg2+ | 0.3-0.4 mM, in the presence of K+ | Prunus persica | |
2.7.1.20 | Mn2+ | at 0.4-0.5 mM, in the presence of K+ | Prunus persica | |
2.7.1.20 | Rb+ | activation, at 100 mM, weaker than K+ | Prunus persica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 45000 | - |
gel filtration | Prunus persica |
2.7.1.20 | 434000 | - |
1 * 434000, SDS-PAGE | Prunus persica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.20 | Prunus persica | - |
Batsch cv Redhaven, peach | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.20 | ammonium sulfate fractionation, gel filtration ion exchange and affinity chromatography | Prunus persica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.20 | flower | buds | Prunus persica | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.20 | -25ºC, variable losses of activity | Prunus persica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.20 | ATP + adenosine | best substrates | Prunus persica | ADP + AMP | - |
? | |
2.7.1.20 | CTP + adenosine | - |
Prunus persica | CDP + AMP | - |
? | |
2.7.1.20 | GTP + adenosine | less efficient than ATP | Prunus persica | GDP + AMP | - |
? | |
2.7.1.20 | UTP + adenosine | - |
Prunus persica | UDP + AMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.20 | monomer | 1 * 434000, SDS-PAGE | Prunus persica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 8.5 | 9.5 | - |
Prunus persica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 8.5 | 9.5 | sharp drop of activity outside the range, especially between pH 6.5 and pH 8.5 | Prunus persica |