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Literature summary extracted from

  • Palella, T.D.; Andres, C.M.; Fox, I.H.
    Human placental adenosine kinase. Kinetic mechanism and inhibition (1980), J. Biol. Chem., 255, 5264-5269.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.20 bovine serum albumin stabilizes Homo sapiens
2.7.1.20 purified enzyme preparation is stable in the concentrated form Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 2',3'-isopropylidene adenosine weak inhibition Homo sapiens
2.7.1.20 2'-deoxyadenosine substrate inhibition Homo sapiens
2.7.1.20 3'-deoxyadenosine
-
 Homo sapiens
2.7.1.20 4-amino-5-iodo-7beta-D-ribofuranosyl-7H-pyrrole(2,3-d)-pyrimidine 100% inhibition Homo sapiens
2.7.1.20 5'-deoxyadenosine 100% inhibition Homo sapiens
2.7.1.20 6-gamma,gamma-dimethylallylaminopurine riboside 90% inhibition Homo sapiens
2.7.1.20 6-Methylmercaptopurine riboside strong inhibition, substrate inhibition Homo sapiens
2.7.1.20 adenosine above 0.0025 mM, at pH 7.4, with ATP and Mg2+ 0.2 mM Homo sapiens
2.7.1.20 ADP noncompetitive with respect to adenosine and ATP Homo sapiens
2.7.1.20 AMP competitive with respect to adenosine and noncompetitive with respect to ATP Homo sapiens
2.7.1.20 ATP
-
 Homo sapiens
2.7.1.20 deoxymethylthioadenosine weak inhibition Homo sapiens
2.7.1.20 Mg2+ at high concentrations Homo sapiens
2.7.1.20 additional information at pH 5.5, with equimolar concentrations of ATP and Mg2+ from 0.2 to 1 mM there is no inhibition by adenosine over a range of 0 to 0.02 mM; product inhibition study Homo sapiens
2.7.1.20 S-adenosylhomocysteine 97% inhibition Homo sapiens
2.7.1.20 uridine weak inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.00015 0.0005 adenosine pH 7.4, 37ºC Homo sapiens
2.7.1.20 0.073 0.0755 ATP pH 7.4, 37ºC Homo sapiens
2.7.1.20 0.073 0.0755 ATP ATP in form of MgATP2- Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ requirement Homo sapiens
2.7.1.20 Mg2+ reactive species: MgATP2- Homo sapiens
2.7.1.20 Mg2+ optimum activity with 0.4-0.8 mM, at pH 6.5. Above, inhibition Homo sapiens
2.7.1.20 Mg2+ free nucleotide or free metal ion exert inhibitory effects Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 3600fold purification Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.20 ATP + adenosine = ADP + AMP mechanism Homo sapiens
a
2.7.1.20 ATP + adenosine = ADP + AMP ordered bi bi mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 2.8
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Homo sapiens ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + adenosine MgATP2- is the true substrate Homo sapiens ADP + AMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 6.5
-
 Mg2+/ATP ratio 1 to 2 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.20 additional information
-
 progressive decrease in activity at pH 7.5 and 8.5, no activity at pH 4.5 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.20 0.14
-
 AMP pH 7.4, 37ºC, at variable adenosine and saturating MgATP2- concentration Homo sapiens
2.7.1.20 0.5
-
 AMP pH 7.4, 37ºC, at variable ATP and saturating adenosine concentration Homo sapiens