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Literature summary extracted from

  • Laht, S.; Karp, H.; Kotka, P.; Jarviste, A.; Alamae, T.
    Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae (2002), Gene, 296, 195-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Hansenula polymorpha A31-10 Ogataea angusta
2.7.1.1 expression in Saccharomyces cerevisiae Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.05
-
D-glucose pH 7.5 Ogataea angusta
2.7.1.1 0.28
-
ATP pH 7.5 Ogataea angusta
2.7.1.2 0.028
-
D-glucose
-
Saccharomyces cerevisiae
2.7.1.2 0.05
-
ATP
-
Saccharomyces cerevisiae
2.7.1.2 0.063
-
D-glucose
-
Aspergillus niger
2.7.1.2 0.21
-
ATP
-
Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 51600
-
calculated from amino acid sequence Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Ogataea angusta
-
ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose Yarrowia lipolytica
-
ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose Saccharomyces cerevisiae
-
ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose Aspergillus niger
-
ADP + D-glucose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Ogataea angusta
-
-
-
2.7.1.2 Aspergillus niger Q92407
-
-
2.7.1.2 Saccharomyces cerevisiae P17709
-
-
2.7.1.2 Yarrowia lipolytica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
Ogataea angusta ADP + 2-deoxy-D-glucose 6-phosphate
-
?
2.7.1.1 ATP + D-glucose
-
Ogataea angusta ADP + D-glucose 6-phosphate
-
?
2.7.1.1 ATP + D-mannose
-
Ogataea angusta ADP + D-mannose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose
-
Yarrowia lipolytica ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose
-
Saccharomyces cerevisiae ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose
-
Aspergillus niger ADP + D-glucose 6-phosphate
-
?