EC Number | General Stability | Organism |
---|---|---|
2.7.1.2 | DTT stabilizes at 70°C | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.2 | 1,10-phenanthroline | - |
Geobacillus stearothermophilus | |
2.7.1.2 | AgNO3 | - |
Geobacillus stearothermophilus | |
2.7.1.2 | D-glucose 6-phosphate | - |
Geobacillus stearothermophilus | |
2.7.1.2 | D-maltose | - |
Geobacillus stearothermophilus | |
2.7.1.2 | HgCl2 | - |
Geobacillus stearothermophilus | |
2.7.1.2 | iodoacetamide | - |
Geobacillus stearothermophilus | |
2.7.1.2 | N-acetyl-alpha-D-glucosamine | - |
Geobacillus stearothermophilus | |
2.7.1.2 | N-ethylmaleimide | - |
Geobacillus stearothermophilus | |
2.7.1.2 | p-chlormercuribenzoate | - |
Geobacillus stearothermophilus | |
2.7.1.2 | p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
2.7.1.2 | Pb(NO3)2 | - |
Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.2 | 0.15 | - |
alpha-D-glucose | 30°C, pH 7.6 | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.2 | Ca2+ | no activation | Geobacillus stearothermophilus | |
2.7.1.2 | Co2+ | 68% of the activity with Mg2+ | Geobacillus stearothermophilus | |
2.7.1.2 | Mg2+ | required | Geobacillus stearothermophilus | |
2.7.1.2 | Mn2+ | 45% of the activity with Mg2+ | Geobacillus stearothermophilus | |
2.7.1.2 | Zn2+ | no activation | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 87000 | - |
HPLC gel filtration | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.2 | ATP + D-glucose | Geobacillus stearothermophilus | - |
ADP + D-glucose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.2 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.2 | large scale | Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 334 | - |
- |
Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.2 | ATP + alpha-D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | ATP + D-glucose | - |
Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | ATP + D-glucose | highly specific for D-glucose | Geobacillus stearothermophilus | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | ATP + N-acetyl-alpha-D-glucosamine | at pH 7.6: 50% of the activity with alpha-D-glucose, at pH 9.0: 5% of the activity with alpha-D-glucose | Geobacillus stearothermophilus | ADP + N-acetyl-alpha-D-glucosamine 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.2 | homodimer | alpha2, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 30 | - |
assay at | Geobacillus stearothermophilus |
2.7.1.2 | 60 | - |
- |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | additional information | - |
DTT stabilizes at 70°C | Geobacillus stearothermophilus |
2.7.1.2 | 70 | - |
complete loss of activity in 70 min in presence of DTT | Geobacillus stearothermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 9 | - |
- |
Geobacillus stearothermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 6 | 10.5 | pH 6.0: about 30% of activity maximum, pH 10.5: about 80% of activity maximum | Geobacillus stearothermophilus |