Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Runquist, J.A.; Rios, S.E.; Vinarov, D.A.; Miziorko, H.M.
    Functional evaluation of serine/threonine residues in the P-loop of Rhodobacter sphaeroides phosphoribulokinase (2001), Biochemistry, 400, 14530-14537.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.19
-
 Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.19 S14A decreased Vmax by 40fold Cereibacter sphaeroides
2.7.1.19 S19A ability to form complexes with Mg-ATP2-, decreased Vmax, increased Km for D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 T18A ability to form complexes with Mg-ATP2-, increased Km for D-ribulose 5-phosphate, decreased Vmax Cereibacter sphaeroides
2.7.1.19 T20A ability to form complexes with Mg-ATP2- Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 6-phospho-D-gluconate
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.096
-
 D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.586
-
 D-ribulose 5-phosphate S14A mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.662
-
 D-ribulose 5-phosphate T20A mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 3.16
-
 D-ribulose 5-phosphate S19A mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 4.5
-
 D-ribulose 5-phosphate T18A mutant, pH 8, 30°C Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 250000
-
 native enzyme Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 octamer 8 *32000, SDS-PAGE Cereibacter sphaeroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.19 0.2
-
 6-phospho-D-gluconate T18A mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.22
-
 6-phospho-D-gluconate wild-type enzyme and S19A mutant, pH 8, 30°C Cereibacter sphaeroides