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Literature summary extracted from

  • Marsden, W.J.N.; Codd, G.A.
    Purification and molecular and catalytic properties of phosphoribulokinase from the cyanobacterium Chlorogloeopsis fritschii (1984), J. Gen. Microbiol., 130, 999-1006.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 dithiothreitol activation in semi-purified extracts Chlorogloeopsis fritschii
2.7.1.19 additional information no activation by dithiothreitol Chlorogloeopsis fritschii
2.7.1.19 additional information not activated by NAD+, NADP+, NADPH, reduced glutathione Chlorogloeopsis fritschii
2.7.1.19 reduced glutathione activation in semi-purified extracts Chlorogloeopsis fritschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 ADP competitive, 1 mM Chlorogloeopsis fritschii
2.7.1.19 AMP competitive, 1 mM Chlorogloeopsis fritschii
2.7.1.19 Ca2+ 200 mM Chlorogloeopsis fritschii
2.7.1.19 Co2+ 200 mM Chlorogloeopsis fritschii
2.7.1.19 L-aspartate competitive, 1 mM Chlorogloeopsis fritschii
2.7.1.19 L-malate competitive, 1 mM Chlorogloeopsis fritschii
2.7.1.19 Mn2+ 200 mM Chlorogloeopsis fritschii
2.7.1.19 phosphoenolpyruvate competitive, 1 mM Chlorogloeopsis fritschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.19 Ca2+ can partially replace Mg2+ Chlorogloeopsis fritschii
2.7.1.19 Ca2+ 5 mM Chlorogloeopsis fritschii
2.7.1.19 Mg2+ required Chlorogloeopsis fritschii
2.7.1.19 Mn2+ can partially replace Mg2+ Chlorogloeopsis fritschii
2.7.1.19 Mn2+ 5 mM Chlorogloeopsis fritschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 40000
-
 6 * 40000, SDS-PAGE Chlorogloeopsis fritschii
2.7.1.19 230000
-
 gel filtration Chlorogloeopsis fritschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Chlorogloeopsis fritschii
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Chlorogloeopsis fritschii
-
 cyanobacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19
-
 Chlorogloeopsis fritschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 14.24
-
-
 Chlorogloeopsis fritschii

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 -20°C, no loss of activity for 4 weeks Chlorogloeopsis fritschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Chlorogloeopsis fritschii D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 hexamer 6 * 40000, SDS-PAGE Chlorogloeopsis fritschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.19 8.6
-
-
 Chlorogloeopsis fritschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.19 NADH no activation Chlorogloeopsis fritschii