Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Porter, M.A.; Milanez, S.; Stringer, C.D.; Hartman, F.C.
    Purification and characterization of ribulose-5-phosphate kinase from spinach (1986), Arch. Biochem. Biophys., 245, 14-23.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 44000
-
 2 * 44000, SDS-PAGE Spinacia oleracea
2.7.1.19 90000
-
 non-denaturing gel electrophoresis Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Spinacia oleracea
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 95% pure Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.19 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 additional information
-
-
 Spinacia oleracea
2.7.1.19 410
-
 buffer containing fresh dithiothreitol Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 -80°C, N2-atmosphere, 50 mM bicine-KOH, 10 mM potassium phosphate, 10 mM dithiothreitol, 1 mM EDTA, 10% v/v glycerol, 2 months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Spinacia oleracea D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 dimer 2 * 44000, SDS-PAGE Spinacia oleracea