Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lin, M.; Turpin, D.H.
    Purification and molecular and immunological characterization of a unique phosphoribulokinase from the green alga Selenastrum minutum (1992), Plant Physiol., 98, 82-88.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 40000
-
 1 * 40000 + 1 * 41000, nonidentical, SDS-PAGE Monoraphidium minutum
2.7.1.19 41000
-
 1 * 40000 + 1 * 41000, nonidentical, SDS-PAGE Monoraphidium minutum
2.7.1.19 82800 83000 gel filtration Monoraphidium minutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Monoraphidium minutum
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Monoraphidium minutum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 homogeneity Monoraphidium minutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 197.5
-
-
 Monoraphidium minutum

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 -80°C, 10 mM bicine-KOH buffer, pH 7.8, 100 mM KCl, 5 mM dithiothreitol, several months Monoraphidium minutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Monoraphidium minutum D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.19 dimer 1 * 40000 + 1 * 41000, nonidentical, SDS-PAGE Monoraphidium minutum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.19 Monoraphidium minutum non-denaturatung isoelectric focusing
-
 5.1