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Literature summary extracted from
- Spinach phosphoribulokinase (1956), J. Biol. Chem., 218, 769-783.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.7.1.19 | unstable to repeated freezing/thawing | Spinacia oleracea |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.19 | Hg2+ | - |
Spinacia oleracea |  |
| 2.7.1.19 | p-chloromercuribenzoate | - |
Spinacia oleracea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.19 | Ca2+ | can partially replace Mg2+ | Spinacia oleracea | |
| 2.7.1.19 | Co2+ | can partially replace Mg2+ | Spinacia oleracea | |
| 2.7.1.19 | EDTA | activation | Spinacia oleracea | |
| 2.7.1.19 | Mg2+ | required | Spinacia oleracea | |
| 2.7.1.19 | Mn2+ | can partially replace Mg2+ | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.19 | D-ribulose 5-phosphate + ATP | Spinacia oleracea | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.19 | Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.19 | - |
Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.1.19 | leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.19 | 310 | - |
- |
Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.1.19 | -10°C, stable | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.19 | D-ribulose 5-phosphate + ATP | - |
Spinacia oleracea | D-ribulose 1,5-diphosphate + ADP | - |
? | |
| 2.7.1.19 | additional information | no activity with fructose 6-phosphate | Spinacia oleracea | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.19 | 7.9 | - |
- |
Spinacia oleracea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.19 | 7 | 8.2 | less than 50% of maximal activity above and below | Spinacia oleracea |
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Release 2023.1 (January 2023)
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