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Literature summary extracted from

  • Lee, N.; Bendet, I.
    Crystalline L-ribulokinase from Escherichia coli (1967), J. Biol. Chem., 242, 2043-2050.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.16
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.16 0.076
-
 ATP 37°C, pH 7.6 Escherichia coli
2.7.1.16 0.111
-
 L-ribulose 37°C, pH 7.6 Escherichia coli
2.7.1.16 0.27
-
 D-ribulose 37°C, pH 7.6 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.16 cytosol
-
 Escherichia coli 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.16 Mg2+ requirement, Km-value: 1.3 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.16 96500 100000 osmotic pressure measurement, ultracentrifugation, ultracentrifugation and diffusion method Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.16 ATP + D-ribulose Escherichia coli
-
 ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + D-ribulose Escherichia coli bB/r
-
 ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose Escherichia coli inducible enzyme of L-arabinose catabolism ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose Escherichia coli bB/r inducible enzyme of L-arabinose catabolism ADP + L-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.16 Escherichia coli
-
 mutant araA-2
-
2.7.1.16 Escherichia coli bB/r
-
 mutant araA-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.16
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.16 833
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.16 ATP + D-ribulose
-
 Escherichia coli ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + D-ribulose
-
 Escherichia coli bB/r ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose no substrates are both isomers of xylulose and other aldopentoses Escherichia coli ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose inducible enzyme of L-arabinose catabolism Escherichia coli ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose no substrates are both isomers of xylulose and other aldopentoses Escherichia coli bB/r ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose inducible enzyme of L-arabinose catabolism Escherichia coli bB/r ADP + L-ribulose 5-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.16 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.16 60.5
-
 at least 30 min stable at pH 7.6 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.16 7.6
-
 and above, stable at least 30 min at 60.5°C Escherichia coli