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Literature summary extracted from

  • Agranoff, B.W.; Brady, R.O.
    Purification and properties of calf liver ribokinase (1956), J. Biol. Chem., 219, 221-229.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.15 Na+ 0.02 M, 30% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.15 0.022
-
 D-ribose 30°C, pH 7 Bos taurus
2.7.1.15 0.035
-
 2-deoxy-D-ribose 30°C, pH 7 Bos taurus
2.7.1.15 0.04
-
 ATP 30°C, pH 7, in presence of Mg2+ Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.15 Co2+
-
 Bos taurus
2.7.1.15 Mg2+ absolute requirement for divalent cations, best satisfied by Mg2+ Bos taurus
2.7.1.15 Mn2+ can replace Mg2+ in activation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.15 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.15
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.15 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.15 23.2
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.15 -12°C fraction IV retains 65% activity after two months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.15 ATP + 2-deoxy-D-ribose
-
 Bos taurus ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose
-
 Bos taurus ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 CTP + D-ribose
-
 Bos taurus CDP + D-ribose 5-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.15 7.1 7.8
-
 Bos taurus