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Literature summary extracted from

  • Ginsburg, A.
    A deoxyribokinase from Lactobacillus plantarum (1959), J. Biol. Chem., 234, 481-487.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.15 EDTA 50 mM Lactiplantibacillus plantarum
2.7.1.15 Mg2+ absolute requirement, optimal ratio of Mg2+ to ATP is 1.5 : 1, higher concentrations inhibit Lactiplantibacillus plantarum
2.7.1.15 Mn2+ 50 mM inhibit Lactiplantibacillus plantarum
2.7.1.15 additional information not: n-ethylmaleimide Lactiplantibacillus plantarum
2.7.1.15 p-chloromercuribenzoate
-
 Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.15 1.8
-
 2-deoxy-D-ribose 37°C, pH 7.2 Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.15 Mg2+ absolute requirement, optimal ratio of Mg2+ to ATP is 1.5 : 1, higher concentrations inhibit Lactiplantibacillus plantarum
2.7.1.15 Mn2+ cannot replace Mg2+ in the kinase reaction, 50 mM inhibit Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.15 ATP + 2-deoxy-D-ribose Lactiplantibacillus plantarum
-
 ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.15 Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.15
-
 Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.15 77
-
 with D-ribose as substrate Lactiplantibacillus plantarum
2.7.1.15 89
-
 with 2-deoxy-D-ribose as substrate Lactiplantibacillus plantarum

Storage Stability

EC Number Storage Stability Organism
2.7.1.15 -10°C gamma gel supernatants lose 64% activity in 3 months Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.15 ATP + 2-deoxy-D-ribose
-
 Lactiplantibacillus plantarum ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose
-
 Lactiplantibacillus plantarum ADP + D-ribose 5-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.15 37
-
 assay at Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.15 60
-
 5 min, complete inactivation Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.15 6.7 7.3 2-deoxy-D-ribose Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.15 5.5 9 pH 5.5: about 45% of activity maximum, pH 9.0: about 55% of activity maximum Lactiplantibacillus plantarum