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Literature summary extracted from

  • Chang, S.C.; Miller, A.L.; Feng, Y.; Wente, S.R.; Majerus, P.W.
    The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase (2002), J. Biol. Chem., 277, 43836-43843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.140 expression in Escherichia coli Homo sapiens
2.7.1.151 DNA and amino acid sequence determination and analysis, no functional complementation of yeast mutant strains, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.140 0.00011
-
 myo-inositol-1,4,5-trisphosphate pH 7.2, 37°C Homo sapiens
2.7.1.140 0.00013
-
 myo-inositol-1,3,4,5-tetrakisphosphate pH 7.2, 37°C Homo sapiens
2.7.1.140 0.0003
-
 myo-inositol-1,3,4,6-tetrakisphosphate pH 7.2, 37°C Homo sapiens
2.7.1.151 additional information
-
 additional information kinetics Homo sapiens
2.7.1.151 0.00011
-
 1D-myo-inositol 1,4,5-trisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens
2.7.1.151 0.00013
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens
2.7.1.151 0.0003
-
 1D-myo-inositol 1,3,4,6-tetrakisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.151 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.140 ATP + myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens
-
 ADP + myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.140 Homo sapiens
-
 human EST
-
2.7.1.151 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.140 recombinant His-tagged enzyme, cobalt-affinity column Homo sapiens
2.7.1.151 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.151 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.140 ATP + myo-inositol 1,3,4,5-tetrakisphosphate approx. 2% of activity with 1,3,4,6-tetrakisphosphate Homo sapiens ADP + myo-inositol-1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.140 ATP + myo-inositol 1,3,4,6-tetrakisphosphate
-
 Homo sapiens ADP + myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.140 ATP + myo-inositol 1,4,5-trisphosphate approx. 50% of activity with 1D-myo-inositol 1,3,4,6-tetrakisphosphate, no activity with 1D-myo-inositol 1,3,4-trisphosphate Homo sapiens ADP + myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate 43fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate preferred substrate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate 2fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate 2fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.151 additional information substrate specificity, product identification Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.151 1,3,4,6-tetrakisphosphate 5-kinase
-
 Homo sapiens
2.7.1.151 inositol phosphate multikinase
-
 Homo sapiens
2.7.1.151 InsP4 5-kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.151 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.151 7.2
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.151 ATP enzyme contains the putative binding site P-X-X-X-D-X-K-X-G Homo sapiens