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Literature summary extracted from

  • Tan, Z.; Bruzik, K.S.; Shears, S.B.
    Properties of the inositol 3,4,5,6-tetrakisphosphate 1-kinase purified from rat liver. Regulation of enzyme activity by inositol 1,3,4-trisphosphate (1997), J. Biol. Chem., 272, 2285-2290.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.134 10 mM sodium diphosphate stabilizes ammonium sulfate precipitated enzyme, no loss of activity after 2 days at 4°C Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.134 inositol 1,3,4-trisphosphate competitive inhibition Rattus norvegicus
2.7.1.134 inositol-1,3,4,5,6-pentakisphosphate competitive inhibition Rattus norvegicus
2.7.1.134 Inositol-1,3,4,5-tetrakisphosphate competitive inhibition Rattus norvegicus
2.7.1.134 Inositol-1,3,4,6-tetrakisphosphate competitive inhibition Rattus norvegicus
2.7.1.134 inositol-1,4,5-trisphosphate competitive inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.134 0.00036
-
 myo-inositol-3,4,5,6-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
2.7.1.134 0.01
-
 ATP pH 7.2, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.134 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.134 Ca2+ activation at 0.0001 mM, 10-20% inhibition at 0.001 mM Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.134 46000
-
 liver enzyme, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.134 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.134 heparin-agarose, phenyl-Sepharose, Mono Q Matrex Blue A Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.134 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.134 -70°C, 1 mg/ml bovine serum albumin, 20% glycerol, 6 months, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.134 0.0002
-
 inositol 1,3,4-trisphosphate pH 7.2, 37°C Rattus norvegicus
2.7.1.134 0.009
-
 Inositol 1,3,4,5-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
2.7.1.134 0.015
-
 Inositol 1,3,4,5,6-pentakisphosphate pH 7.2, 37°C Rattus norvegicus
2.7.1.134 0.015
-
 Inositol 1,3,4,6-tetrakisphosphate pH 7.2, 37°C Rattus norvegicus
2.7.1.134 0.048
-
 Inositol 1,4,5-trisphosphate pH 7.2, 37°C Rattus norvegicus