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Literature summary extracted from

  • Chen, C.K.; Inglese, J.; Lefkowitz, R.J.; Hurley, J.B.
    Ca2+-dependent interaction of recoverin with rhodopsin kinase (1995), J. Biol. Chem., 270, 18060-18066.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 rhodopsin photolyzed rhodopsin stimulates Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 RK is cloned and expressed in SF9 cells Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 recoverin Ca2+-dependent inhibition; highly specific direct Ca2+-dependent interaction with RK, N-terminal myristoyl residue of recoverin enhances RK inhibition and introduces cooperativity to the inhibitory effect, quenches high-gain phosphorylation of rhodopsin in the presence of Ca2+ Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Bos taurus
2.7.11.14 additional information Ca2+-independent phosphorylation of rhodopsin Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 64000
-
 x * 64000, SDS-PAGE Bos taurus
2.7.11.14 66000
-
 x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus specific and Ca2+-dependent recoverin/RK interaction may play an important role in photoreceptor light adaptation ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein enzyme is autophosphorylated Bos taurus
2.7.11.14 side-chain modification purified retinal RK is farnesylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 native and recombinant RK, expressed in SF9 cells, recovering affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin recombinant RK expressed in SF9 cells catalyzes high-gain phosphorylation in which photoactivation of one rhodopsin molecule causes incorporation of up to several hundred phosphates into the total rhodopsin pool Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin specific and Ca2+-dependent recoverin/RK interaction may play an important role in photoreceptor light adaptation Bos taurus ADP + phosphorhodopsin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ? x * 64000, SDS-PAGE Bos taurus
2.7.11.14 ? x * 66000, autophosphorylated RK, SDS-PAGE Bos taurus