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Literature summary extracted from

  • Bruel, C.; Cha, K.; Reeves, P.J.; Getmanova, E.; Khorana, H.G.
    Rhodopsin kinase: Expression in mammalian cells and a two-step purification (2000), Proc. Natl. Acad. Sci. USA, 97, 3004-3009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 RK gene is cloned and expressed in Pichia pastoris GS115, COS-1 cells and HEK-293 stable cell line, best in COS-1 cells with correct posttranslational modifications Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.14 glycerol does not protect against inactivation during purification Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein enzyme is autophosphorylated Bos taurus
2.7.11.14 phosphoprotein recombinant RK expressed in COS-1 cells is mainly diphosphorylated Bos taurus
2.7.11.14 side-chain modification enzyme is isoprenylated, recombinant RK expressed in COS-1 cells is mainly farnesylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 recombinant RK with N-terminal hexahistidine tag, expressed in Pichia pastoris GS115, COS-1 cells: 210-222fold, and HEK-293 cell line Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.04
-
 pH 7.4, 30°C, recombinant RK expressed in COS-1 cells, purified in absence or presence of ATP Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 6
-
 His-tagged recombinant RK expressed in COS-1 cells Bos taurus
2.7.11.14 6.67
-
 RK from rod outer segments Bos taurus
2.7.11.14 7.4
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.14 6.67
-
 RK from rod outer segments: 60% of activity is lost with one pH unit change, recombinant RK expressed in COS-1 cells shows broader activity profile Bos taurus