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Literature summary extracted from

  • Cai, K.; Klein-Seetharaman, J.; Hwa, J.; Hubbell, W.L.; Khorana, H.G.
    Structure and function in rhodopsin: Effects of disulfide cross-links in the cytoplasmic face of rhodopsin on transducin activation and phosphorylation by rhodopsin kinase (1999), Biochemistry, 38, 12893-12898.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 RK gene expression in baculovirus-infected Sf21 cells Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 recombinant RK purified from baculovirus-infected Sf21 cells Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: light-activated form of rhodopsin, i.e. metarhodopsin II Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin effect of bovine rhodopsin mutants with disulfide cross-links between different cytoplasmic regions on the possibility to serve as substrate, only substrate is a rhodopsin mutant containing a disulfide cross-link between Cys-65 and Cys-316 Bos taurus ADP + phosphorhodopsin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus