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Literature summary extracted from
- Purification of rhodopsin kinase by recoverin affinity chromatography (2000), Methods Enzymol., 315, 404-410.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.11.14 | RK is cloned and expressed in SF9 cells | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.14 | recoverin | Ca2+-dependent inhibition | Bos taurus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.14 | Mg2+ | - |
Bos taurus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.11.14 | ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.11.14 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.11.14 | native and recombinant RK, expressed in SF9 cells, recovering affinity chromatography | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.11.14 | eye | retina | Bos taurus | - |
| 2.7.11.14 | retina | rod cell outer segment | Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.14 | 1.2 | - |
recombinant RK expressed in SF9 cells | Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.11.14 | -70°C, several months, stable | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.11.14 | ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| 2.7.11.14 | ATP + rhodopsin | recombinant RK expressed in SF9 cells catalyzes high-gain phosphorylation in which photoactivation of one rhodopsin molecule causes incorporation of up to several hundred phosphates into the total rhodopsin pool | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| 2.7.11.14 | ATP + rhodopsin | fully bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| 2.7.11.14 | ATP + rhodopsin | high-gain phosphorylation of rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
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