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Literature summary extracted from

  • Onorato, J.J.; Palczewski, K.; Regan, J.W.; Caron, M.G.; Lefkowitz, R.J.; Benovic, J.L.
    Role of acidic amino acids in peptide substrates of the beta-adrenergic receptor kinase and rhodopsin kinase (1991), Biochemistry, 30, 5118-5125.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 isoproterenol requirement, beta-agonist, beta-adrenergic receptor as substrate Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.15 Triton X-100 stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 heparin
-
 Bos taurus
2.7.11.15 peptide synthetic peptides from a variety of intracellular regions of beta2-AR specifically inhibit phosphorylation of the intact receptor, but not of peptide substrates Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.15 0.9 1.3 peptide acid-rich synthetic peptides Bos taurus
2.7.11.15 1.5 4.8 peptide synthetic peptides containing a single Glu-residue Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 cytosol cytosolic protein Bos taurus 5829
-
2.7.11.14 membrane binds to photobleached rod outer segment membranes Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Bos taurus
2.7.11.15 Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.11.15 Triton X-100 stabilizes Bos taurus
2.7.11.15 Triton X-100 0.02% Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-
2.7.11.15 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.15 ATP + [beta-adrenergic receptor] = ADP + phospho-[beta-adrenergic receptor] mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-
2.7.11.15 brain cerebral cortex Bos taurus
-
2.7.11.15 cerebral cortex
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.5 0.85
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + peptide acid-rich peptides, RK prefers acid residues localized to the C-terminal side of the serine Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: light-activated form of rhodopsin, i.e. metarhodopsin II Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin rhodopsin with multiphosphorylation sites Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + alpha2-adrenergic receptor from human platelets Bos taurus ADP + phospho-alpha2-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor phosphorylation sites Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor incorporation of 6-8 mol phosphate/mol receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + LEESSSSDHAERPPG
-
 Bos taurus ?
-
 ?
2.7.11.15 ATP + muscarinic cholinergic receptor from chick heart Bos taurus ADP + phospho-muscarinic cholinergic receptor
-
 ?
2.7.11.15 ATP + peptide e.g. Leu-Glu-Glu-Ser-Ser-Ser-Ser-Asp-His-Ala-Glu-Arg-Pro-Pro-Gly or Arg-Arg-Arg-Glu-Glu-Glu-Glu-Glu-Ser-Ala-Ala-Ala, role of acidic amino acids in peptide substrates, preference for negatively charged amino acids localized to the N-terminal side of a Ser- or Thr-residue, Ser-containing peptides are 4fold better than Thr-containing Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.15 ATP + peptide synthetic Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.15 ATP + rhodopsin metarhodopsin II, poor substrate Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + RRREEEEESAAA
-
 Bos taurus ?
-
 ?
2.7.11.15 additional information phosphorylates G protein coupled receptors in an agonist-dependent manner Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
 assay at Bos taurus
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.4
-
 assay at Bos taurus
2.7.11.15 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.14 additional information
-
 additional information values for synthetic peptides Bos taurus