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Literature summary extracted from

  • Palczewski, K.; McDowell, J.H.; Hargrave, P.A.
    Purification and characterization of rhodopsin kinase (1988), J. Biol. Chem., 263, 14067-14073.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 D-myo-Inositol 1-phosphate about 20% activation, between 0.0003 mM and 0.1 mM, slightly inhibitory at higher concentrations Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.14 dilution inactivates Bos taurus
2.7.11.14 freezing at liquid N2 temperatures or -20°C, in water or inositol leads to 80% loss of activity, in sucrose to 20% loss of activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 2',3'-dideoxyadenosine
-
 Bos taurus
2.7.11.14 3'-deoxyadenosine
-
 Bos taurus
2.7.11.14 5'-AMP competitive inhibition with respect to ATP Bos taurus
2.7.11.14 5'-deoxyadenosine
-
 Bos taurus
2.7.11.14 adenosine competitive inhibition with respect to ATP Bos taurus
2.7.11.14 adenosine 5'-monosulfate
-
 Bos taurus
2.7.11.14 ADP competitive inhibition with respect to ATP Bos taurus
2.7.11.14 ATP-analogues weak or no inhibition, overview Bos taurus
2.7.11.14 cAMP weak Bos taurus
2.7.11.14 D-myo-Inositol 1-phosphate weak, above 0.1 mM, stimulates below Bos taurus
2.7.11.14 inositol triphosphate weak Bos taurus
2.7.11.14 Mg2+ at high concentrations; requirement at lower concentrations Bos taurus
2.7.11.14 additional information little inhibition by S-adenosyl-L-methionine, coenzyme A, methylene adenosine 5'-triphosphate, imidoadenosine 5'-triphosphate, adenosine 9-arabinofuranoside 5'-monophosphate, GMP, adenosine N1-oxide; not inhibited by 2'-deoxyadenosine, adenosine 2',3'-monophosphate, adenosine 2'-deoxy-3,5-monophosphate, inosine monophosphate, guanosine, GDP, xanthosine 5'-monophosphate, hypoxanthine 9-arabinofuranoside, 1-N6-ethenoadenosine monophosphate, NAD+, NADH, NADP+, NADPH; not inhibited by cAMP; not inhibited by cGMP Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.14 0.0016 0.002 ATP pH 7.5, 25°C, at 1 mM Mg2+ Bos taurus
2.7.11.14 0.004 0.0044 rhodopsin pH 7.5, 25°C, freshly bleached Bos taurus
2.7.11.14 0.0052
-
 ATP pH 7.5, 25°C, at 10 mM Mg2+ Bos taurus
2.7.11.14 1
-
 GTP pH 7.5, 25°C, at 2 mM Mg2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 soluble behaves as a soluble protein Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus
2.7.11.14 Mg2+ inhibition at high concentrations Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 67000
-
 gel filtration Bos taurus
2.7.11.14 70000
-
 1 * 70000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.11.14 Ethanol marked sensitivity to organic solvents, e.g. 5% ethanol reduces the activity by 45% within 2-3 min Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 1055fold, to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.96
-
 pH 7.5, 25°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.14 -20°C, 20% adonitol, several months, stable Bos taurus
2.7.11.14 -20°C, kinase solution, solid sucrose, initially loses 30% of activity, stable to further storage Bos taurus
2.7.11.14 4°C, crude extract, t1/2: 30 days, highly purified enzyme, t1/2: 3-5 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin specificity of ATP-binding site Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: ATP Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 GTP + rhodopsin very poor substrate Bos taurus GDP + phosphorhodopsin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 monomer 1 * 70000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.14 0.004
-
 adenosine pH 7.5, 25°C Bos taurus
2.7.11.14 0.005
-
 5'-AMP pH 7.5, 25°C Bos taurus
2.7.11.14 0.01
-
 5'-deoxyadenosine pH 7.5, 25°C Bos taurus
2.7.11.14 0.012
-
 ADP pH 7.5, 25°C Bos taurus
2.7.11.14 0.016
-
 3'-deoxyadenosine pH 7.5, 25°C Bos taurus
2.7.11.14 0.065
-
 2',3'-dideoxyadenosine pH 7.5, 25°C Bos taurus
2.7.11.14 0.08
-
 adenosine 5'-monosulfate pH 7.5, 25°C Bos taurus