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Literature summary extracted from

  • Shichi, H.; Somers, R.L.
    Light-dependent phosphorylation of rhodopsin. Purification and properties of rhodopsin kinase (1978), J. Biol. Chem., 253, 7040-7046.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 spermidine slight stimulation Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.14 monovalent cations, e.g. K+ or NH4+, and 15% glycerol stabilize to some extent Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 5'-AMP 1 mM, 50% inhibition Bos taurus
2.7.11.14 adenosine 1 mM, 50% inhibition Bos taurus
2.7.11.14 Digitonin 0.1%, 50% inhibition Bos taurus
2.7.11.14 emulphogene 0.1%, 50% inhibition Bos taurus
2.7.11.14 additional information not inhibited by cAMP; not inhibited by cGMP; not inhibited by K+ Bos taurus
2.7.11.14 Na+ 0.1 M, 90% inhibition Bos taurus
2.7.11.14 Zn2+ 1 mM, 90% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.14 0.008
-
 ATP pH 7.4, 37°C Bos taurus
2.7.11.14 0.4
-
 GTP pH 7.4, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane membrane-bound Bos taurus 16020
-
2.7.11.14 membrane membrane-bound Lithobates pipiens 16020
-
2.7.11.14 membrane phosphorylation occurs preferentially in newly formed discs Bos taurus 16020
-
2.7.11.14 membrane phosphorylation occurs preferentially in newly formed discs Lithobates pipiens 16020
-
2.7.11.14 membrane rod membranes Bos taurus 16020
-
2.7.11.14 membrane rod membranes Lithobates pipiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus
2.7.11.14 Mg2+ requirement Lithobates pipiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 50000
-
 1 * 50000, SDS-PAGE Bos taurus
2.7.11.14 53000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus enzyme in vivo is probably inactive in the dark, but is almost fully activated in the light ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Lithobates pipiens enzyme in vivo is probably inactive in the dark, but is almost fully activated in the light ADP + phosphorhodopsin
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.11.14 urea 5 M, almost complete denaturation of enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-
2.7.11.14 Lithobates pipiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 87-110fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 eye retina Lithobates pipiens
-
2.7.11.14 eye dark-adapted Bos taurus
-
2.7.11.14 eye dark-adapted Lithobates pipiens
-
2.7.11.14 retina rod cell outer segment Bos taurus
-
2.7.11.14 retina rod cell outer segment Lithobates pipiens
-
2.7.11.14 retina from dark-adapted eyes Bos taurus
-
2.7.11.14 retina from dark-adapted eyes Lithobates pipiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.026 0.033
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.14 3°C, 0.1 M KCl, 1 week, 50% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin phosphorylates rhodopsin in the disc-membrane Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin phosphorylates rhodopsin in the disc-membrane Lithobates pipiens ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin highly specific for rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin highly specific for rhodopsin Lithobates pipiens ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin preferred substrate: ATP Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin rhodopsin with multiphosphorylation sites Bos taurus ADP + phosphorhodopsin
-
 ir
2.7.11.14 ATP + rhodopsin enzyme in vivo is probably inactive in the dark, but is almost fully activated in the light Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin enzyme in vivo is probably inactive in the dark, but is almost fully activated in the light Lithobates pipiens ADP + phosphorhodopsin
-
 ?
2.7.11.14 GTP + rhodopsin can replace ATP to a lesser extent Bos taurus GDP + phosphorhodopsin
-
 ?
2.7.11.14 additional information not: protamine Bos taurus ?
-
 ?
2.7.11.14 additional information not: casein, phosvitin, histones Bos taurus ?
-
 ?
2.7.11.14 additional information not: casein, phosvitin, histones Lithobates pipiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 monomer 1 * 50000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 37
-
 assay at Bos taurus
2.7.11.14 37
-
 assay at Lithobates pipiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.14 additional information
-
 additional information ATP: 2.2 mol of phosphate bound/min, GTP: 0.12 mol of phosphate bound/min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.4
-
 assay at Bos taurus