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Literature summary extracted from

  • Green, F.J.; Lewis, R.A.
    Partial purification and characterization of deoxyguanosine kinase from pig skin (1979), Biochem. J., 183, 547-553.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.113 AMP weak inhibition Sus scrofa
2.7.1.113 ATP weak inhibition Sus scrofa
2.7.1.113 CMP weak inhibition Sus scrofa
2.7.1.113 dATP 0.1 mM, 62% inhibition Sus scrofa
2.7.1.113 dCMP weak inhibition Sus scrofa
2.7.1.113 dCTP 0.1 mM, 48% inhibition Sus scrofa
2.7.1.113 deoxyadenosine weak inhibition Sus scrofa
2.7.1.113 dGMP 0.1 mM, 44% inhibition Sus scrofa
2.7.1.113 dGTP 0.1 mM, complete inhibition Sus scrofa
2.7.1.113 dTMP weak inhibition Sus scrofa
2.7.1.113 dTTP 0.1 mM, 30% inhibition Sus scrofa
2.7.1.113 GMP weak inhibition Sus scrofa
2.7.1.113 GTP 0.1 mM, 33% inhibition Sus scrofa
2.7.1.113 additional information not inhibited by dAMP Sus scrofa
2.7.1.113 UMP 0.1 mM, 24% inhibition Sus scrofa
2.7.1.113 UTP 0.1 mM, 30% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.113 0.00032
-
 deoxyguanosine pH 8.0, 37°C Sus scrofa
2.7.1.113 3.3
-
 ATP pH 8.0, 37°C Sus scrofa
2.7.1.113 3.3
-
 ATP ATP in form of MgATP2- Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.113 Ca2+ activation Sus scrofa
2.7.1.113 Ca2+ 67% of activity with Mg2+ Sus scrofa
2.7.1.113 Fe2+ slight activation Sus scrofa
2.7.1.113 Mg2+ required for activity Sus scrofa
2.7.1.113 Mn2+ 92% of activity with Mg2+ Sus scrofa
2.7.1.113 additional information divalent cations required, not activated by Zn2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.113 58500
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.113 Sus scrofa
-
 piglet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.113 pH 5.5, ammonium sulfate, DEAE-Sephadex Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.113 skin
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.113 0.0000338
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.113 ATP + deoxyguanosine highly specific for phospho group acceptor Sus scrofa ADP + dGMP
-
 ?
2.7.1.113 ATP + deoxyguanosine ATP twice as effective as CTP or GTP and 4 times as effective as UTP Sus scrofa ADP + dGMP
-
 ?
2.7.1.113 CTP + deoxyguanosine 50% of activity with ATP Sus scrofa CDP + dGMP
-
 ?
2.7.1.113 GTP + deoxyguanosine 50% of activity with ATP Sus scrofa GDP + dGMP
-
 ?
2.7.1.113 UTP + deoxyguanosine 25% of activity with ATP Sus scrofa UDP + dGMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.113 37
-
-
 Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.113 30 53 17% loss of activity at 53°C, 50% loss of activity at 30°C and 53°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.113 8.5
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.113 5 9 approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.113 0.021
-
 dGTP pH 8.0, 37°C Sus scrofa
2.7.1.113 0.076
-
 dGMP pH 8.0, 37°C Sus scrofa
2.7.1.113 0.34
-
 deoxyadenosine pH 8.0, 37°C Sus scrofa