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Literature summary extracted from

  • Wang, X.; Kemp, R.G.
    Reaction path of phosphofructo-1-kinase is altered by mutagenesis and alternative substrates (2001), Biochemistry, 40, 3938-3942.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.11 M169A 142fold lower kcat than wild-type Escherichia coli
2.7.1.11 M169L 6fold lower kcat than wild-type Escherichia coli
2.7.1.11 R25S not activated by GDP, pattern of ATP inhibition nearly identical to wild-type Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP inhibition of fructose 1-phosphate phosphorylation above 0.08 mM ATP Escherichia coli
2.7.1.11 additional information no substrate inhibition with gamma-thio-ATP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.02
-
ATP pH 7.2, 30°C, cosubstrate fructose 1-phosphate Escherichia coli
2.7.1.11 0.096
-
D-fructose 6-phosphate pH 7.2, 30°C, cosubstrate gamma-thio-ATP Escherichia coli
2.7.1.11 0.1
-
ATP pH 7.2, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Escherichia coli P0A796
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 1-carboxymethyl-ATP + D-fructose 6-phosphate 90% of activity with with ATP Escherichia coli 1-carboxymethyl-ADP + D-fructose 1,6-bisphosphate
-
?
2.7.1.11 ATP + fructose 1-phosphate 60fold lower kcat than with fructose 6-phosphate Escherichia coli ADP + fructose 1,6-bisphosphate
-
?
2.7.1.11 gamma-thio-ATP + fructose 6-phosphate 3000fold lower kcat than with ATP Escherichia coli ADP + fructose 1-thio-phosphate-6-phosphate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.11 0.016
-
D-fructose 6-phosphate pH 7.2, 30°C, cosubstrate gamma-thio-ATP Escherichia coli
2.7.1.11 49
-
fructose 6-phosphate pH 7.2, 30°C, cosubstrate ATP Escherichia coli
2.7.1.11 88
-
fructose 6-phosphate pH 7.2, 30°C, at 2mM ATP Escherichia coli