Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hansen, T.; Musfeldt, M.; Schonheit, P.
    ATP-dependent 6-phosphofructokinase from the hyperthermophilic bacterium Thermotoga maritima: characterization of an extremely thermophilic, allosterically regulated enzyme (2002), Arch. Microbiol., 177, 401-409.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP
-
 Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 phosphoenolpyruvate 0.1 mM and 10 mM, allosteric inhibition, almost completely reversed by 2.5 mM ADP Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.2
-
 ATP pH 7.0, 50°C and 75°C Thermotoga maritima
2.7.1.11 1.4
-
 ADP pH 7.0, 75°C Thermotoga maritima
2.7.1.11 7.6
-
 D-fructose 1,6-bisphosphate pH 7.0, 75°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Fe2+ 30% of activity with Mg2+ Thermotoga maritima
2.7.1.11 Mg2+ required for activity Thermotoga maritima
2.7.1.11 Mn2+
-
 Thermotoga maritima
2.7.1.11 Zn2+ 70% of activity with Mg2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 34000
-
 alpha4, 4 * 34000, SDS-PAGE Thermotoga maritima
2.7.1.11 140000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate Thermotoga maritima
-
 ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 Q-Sepharose, ATP-agarose, Uno S1 Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 62
-
 at 50°C Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate specific for fructose 6-phosphate, very low activity with UTP and CTP Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate reversible reaction in vitro Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 GTP + D-fructose 6-phosphate 64% of activity of ATP Thermotoga maritima GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate 38% of activity with ATP Thermotoga maritima IDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 tetramer alpha4, 4 * 34000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 93
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.11 20 100 very low activity at 20°C, exponential increase in activity between 40°C and 93°C, approx. 50% of maximal activity at 80°C, activation energy: 54 kJ/mol Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 70
-
 less than 20% loss of activity after 2 h Thermotoga maritima
2.7.1.11 80
-
 less than 20% loss of activity after 2 h Thermotoga maritima
2.7.1.11 95
-
 complete loss of activity after 1 h Thermotoga maritima
2.7.1.11 100
-
 complete loss of activity after 20 min, 40% loss of activity after 2 h in the presence of 1 M ammonium sulfate Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 6.7
-
 forward direction, at 75°C, 50% of maximal activity at pH 5.7, 80% at pH 7.7 Thermotoga maritima