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Literature summary extracted from

  • Baumann, L.; Baumann, P.
    Catabolism of D-fructose and D-ribose by Pseudomonas doudoroffii. II. Properties of 1-phosphofructokinase and 6-phosphofructokinase (1975), Arch. Microbiol., 105, 241-248.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP activation Oceanimonas doudoroffii
2.7.1.11 ADP positive effector Oceanimonas doudoroffii
2.7.1.11 NH4+ required for activity Oceanimonas doudoroffii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ATP above 0.25 mM in the absence of MgCl2; free form Oceanimonas doudoroffii
2.7.1.11 citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Oceanimonas doudoroffii
2.7.1.11 additional information not inhibited by AMP; not inhibited by cAMP; not inhibited by phosphate; not inhibited by pyruvate Oceanimonas doudoroffii
2.7.1.11 phosphoenolpyruvate
-
 Oceanimonas doudoroffii
2.7.1.56 ADP ATP reverses Oceanimonas doudoroffii
2.7.1.56 ATP at a Mg2+/ATP ratio below 2; free form Oceanimonas doudoroffii
2.7.1.56 K+ 300 mM, activates at 30 mM Oceanimonas doudoroffii
2.7.1.56 additional information not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by CTP and UTP; not inhibited by D-fructose 6-phosphate; not inhibited by L-glutamate; not inhibited by phosphate, AMP Oceanimonas doudoroffii
2.7.1.56 NaCl
-
 Oceanimonas doudoroffii
2.7.1.56 SO42- above 25 mM Oceanimonas doudoroffii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.14
-
 ATP pH 8.0, 28°C Oceanimonas doudoroffii
2.7.1.11 0.15
-
 ATP
-
 Oceanimonas doudoroffii
2.7.1.56 0.3
-
 D-Fructose 1-phosphate
-
 Oceanimonas doudoroffii
2.7.1.56 0.34
-
 ATP
-
 Oceanimonas doudoroffii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 K+ required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP Oceanimonas doudoroffii
2.7.1.11 Mg2+ required for activity Oceanimonas doudoroffii
2.7.1.11 Mg2+ 1-10 mM are required for maximal activity Oceanimonas doudoroffii
2.7.1.11 Mn2+ as effective as Mg2+ Oceanimonas doudoroffii
2.7.1.11 Mn2+ activation Oceanimonas doudoroffii
2.7.1.56 K+ activation at 30 mM, inhibiton at 300 mM Oceanimonas doudoroffii
2.7.1.56 Mg2+ required for activity Oceanimonas doudoroffii
2.7.1.56 Mg2+ can replace Mn2+ Oceanimonas doudoroffii
2.7.1.56 Mg2+ actual substrate: MgATP2- Oceanimonas doudoroffii
2.7.1.56 Mn2+ required for activity Oceanimonas doudoroffii
2.7.1.56 additional information not activated by Na+ Oceanimonas doudoroffii
2.7.1.56 NH4+ stimulation Oceanimonas doudoroffii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Oceanimonas doudoroffii
-
-
-
2.7.1.11 Oceanimonas doudoroffii M1
-
-
-
2.7.1.56 Oceanimonas doudoroffii
-
 wild-type strain 75
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 partial Oceanimonas doudoroffii
2.7.1.56 partial Oceanimonas doudoroffii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 2.5 3.1
-
 Oceanimonas doudoroffii
2.7.1.56 4.2
-
-
 Oceanimonas doudoroffii

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -15°C, 3 days, complete loss of activity Oceanimonas doudoroffii
2.7.1.11 4°C, 1 week, 47% loss of activity Oceanimonas doudoroffii
2.7.1.56 -15°C, 3 days, 70% loss of activity Oceanimonas doudoroffii
2.7.1.56 4°C, 1 week, about 30% loss of activity Oceanimonas doudoroffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Oceanimonas doudoroffii ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Oceanimonas doudoroffii M1 ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate phosphorylation at 28% the rate of ATP Oceanimonas doudoroffii CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate phosphorylation at 28% the rate of ATP Oceanimonas doudoroffii M1 CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate phosphorylation at 35% the rate of ATP Oceanimonas doudoroffii GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate phosphorylation at 35% the rate of ATP Oceanimonas doudoroffii M1 GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate phosphorylation at 71% the rate of ATP Oceanimonas doudoroffii IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ITP + D-fructose 6-phosphate phosphorylation at 71% the rate of ATP Oceanimonas doudoroffii M1 IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate phosphorylation at 16% the rate of ATP Oceanimonas doudoroffii UDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 UTP + D-fructose 6-phosphate phosphorylation at 16% the rate of ATP Oceanimonas doudoroffii M1 UDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Oceanimonas doudoroffii ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate 69% of activity with ATP Oceanimonas doudoroffii GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ITP + D-fructose 1-phosphate 45% of activity with ATP Oceanimonas doudoroffii IDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.56 28
-
 assay at Oceanimonas doudoroffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 8.5
-
-
 Oceanimonas doudoroffii
2.7.1.56 9
-
-
 Oceanimonas doudoroffii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 7 8 approx. 60% of maximal activity at pH 7, approx 95% of maximal activity at pH 8 Oceanimonas doudoroffii
2.7.1.56 7 8 44% of maximal activity at pH 7 and 81% of maximal activity at pH 8 Oceanimonas doudoroffii